caa a22 4500 445325283 CHVBK 20190328074407.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10858-011-9572-7 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9572-7 4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins [Elektronische Daten] [Barbara Krähenbühl, Sebastian Hiller, Gerhard Wider] A four-dimensional (4D) APSY (automated projection spectroscopy)-HBCB(CG)CDHD experiment is presented. This 4D experiment correlates aromatic with aliphatic carbon and proton resonances from the same amino acid side chain of proteins in aqueous solution. It thus allows unambiguous sequence-specific assignment of aromatic amino acid ring signals based on backbone assignments. Compared to conventional 2D approaches, the inclusion of evolution periods on 1Hβ and 13Cδ efficiently removes overlaps, and provides two additional frequencies for consequent automated or manual matching. The experiment was successfully applied to three proteins with molecular weights from 6 to 13kDa. For the complementation of the assignment of the aromatic resonances, TOCSY- or COSY-based versions of a 4D APSY-HCCHaro sequence are proposed. Springer Science+Business Media B.V., 2011 Protein NMR nationallicence Aromatic resonances nationallicence Projection spectroscopy nationallicence APSY nationallicence GAPRO nationallicence Automated assignment nationallicence Krähenbühl Barbara Institute of Molecular Biology and Biophysics, ETH Zurich, 8093, Zurich, Switzerland aut Hiller Sebastian Biozentrum, University of Basel, 4056, Basel, Switzerland aut Wider Gerhard Institute of Molecular Biology and Biophysics, ETH Zurich, 8093, Zurich, Switzerland aut Journal of Biomolecular NMR Springer Netherlands 51/3(2011-11-01), 313-318 0925-2738 51:3<313 2011 51 10858 https://doi.org/10.1007/s10858-011-9572-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9572-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krähenbühl Barbara Institute of Molecular Biology and Biophysics, ETH Zurich, 8093, Zurich, Switzerland aut NATIONALLICENCE 700 1- Hiller Sebastian Biozentrum, University of Basel, 4056, Basel, Switzerland aut NATIONALLICENCE 700 1- Wider Gerhard Institute of Molecular Biology and Biophysics, ETH Zurich, 8093, Zurich, Switzerland aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/3(2011-11-01), 313-318 0925-2738 51:3<313 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer