caa a22 4500 445325313 CHVBK 20180317142718.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10858-011-9574-5 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9574-5 Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks [Elektronische Daten] [Ophélie Fliniaux, Gwenaelle Gaillard, Antoine Lion, Dominique Cailleu, François Mesnard, Fotini Betsou] A blood pre-centrifugation delay of 24h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24h at 4°C did not influence the spectroscopic profile as compared with 4h delays at either room temperature or 4°C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum. Springer Science+Business Media B.V., 2011 Serum nationallicence Proton NMR nationallicence Preanalytical variation nationallicence Biobank nationallicence Biomarker nationallicence Biospecimen research nationallicence Metabolic profiling nationallicence SST : Serum separation tube nationallicence SPREC : Standard preanalytical code nationallicence TSP : 3-(trimethylsilyl) 3,3,3,3-tetradeutero-propionic acid nationallicence CPMG : Carr-Purcell-Meiboom-Gill nationallicence HSQC : Heteronuclear single quantum coherence nationallicence Fliniaux Ophélie Laboratoire de Phytotechnologie EA 3900-BioPI, University of Picardie Jules Verne, Amiens cedex 1, 80037, Amiens, France aut Gaillard Gwenaelle Biobanque de Picardie, Avenue René Laënnec, 80480, Salouel, France aut Lion Antoine Laboratoire de Phytotechnologie EA 3900-BioPI, University of Picardie Jules Verne, Amiens cedex 1, 80037, Amiens, France aut Cailleu Dominique Plateforme Analytique, Bâtiment Serres-Transfert, rue de Mai/rue Dallery, 80039, Amiens cedex 1, France aut Mesnard François Laboratoire de Phytotechnologie EA 3900-BioPI, University of Picardie Jules Verne, Amiens cedex 1, 80037, Amiens, France aut Betsou Fotini Integrated Biobank of Luxembourg, 6 rue Ernest Barble, 1210, Luxembourg, Luxembourg aut Journal of Biomolecular NMR Springer Netherlands 51/4(2011-12-01), 457-465 0925-2738 51:4<457 2011 51 10858 https://doi.org/10.1007/s10858-011-9574-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9574-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fliniaux Ophélie Laboratoire de Phytotechnologie EA 3900-BioPI, University of Picardie Jules Verne, Amiens cedex 1, 80037, Amiens, France aut NATIONALLICENCE 700 1- Gaillard Gwenaelle Biobanque de Picardie, Avenue René Laënnec, 80480, Salouel, France aut NATIONALLICENCE 700 1- Lion Antoine Laboratoire de Phytotechnologie EA 3900-BioPI, University of Picardie Jules Verne, Amiens cedex 1, 80037, Amiens, France aut NATIONALLICENCE 700 1- Cailleu Dominique Plateforme Analytique, Bâtiment Serres-Transfert, rue de Mai/rue Dallery, 80039, Amiens cedex 1, France aut NATIONALLICENCE 700 1- Mesnard François Laboratoire de Phytotechnologie EA 3900-BioPI, University of Picardie Jules Verne, Amiens cedex 1, 80037, Amiens, France aut NATIONALLICENCE 700 1- Betsou Fotini Integrated Biobank of Luxembourg, 6 rue Ernest Barble, 1210, Luxembourg, Luxembourg aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/4(2011-12-01), 457-465 0925-2738 51:4<457 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer