caa a22 4500 445325372 CHVBK 20180317142718.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10858-011-9577-2 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9577-2 Paramagnetic relaxation enhancement to improve sensitivity of fast NMR methods: application to intrinsically disordered proteins [Elektronische Daten] [François-Xavier Theillet, Andres Binolfi, Stamatis Liokatis, Silvia Verzini, Philipp Selenko] We report enhanced sensitivity NMR measurements of intrinsically disordered proteins in the presence of paramagnetic relaxation enhancement (PRE) agents such as Ni2+-chelated DO2A. In proton-detected 1H-15N SOFAST-HMQC and carbon-detected (H-flip)13CO-15N experiments, faster longitudinal relaxation enables the usage of even shorter interscan delays. This results in higher NMR signal intensities per units of experimental time, without adverse line broadening effects. At 40mmol·L−1 of the PRE agent, we obtain a 1.7- to 1.9-fold larger signal to noise (S/N) for the respective 2D NMR experiments. High solvent accessibility of intrinsically disordered protein (IDP) residues renders this class of proteins particularly amenable to the outlined approach. Springer Science+Business Media B.V., 2011 Intrinsically disordered proteins nationallicence Alpha-synuclein nationallicence Histone H3 nationallicence Paramagnetic relaxation enhancement nationallicence Direct carbon detection nationallicence SOFAST-HMQC nationallicence Theillet François-Xavier Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut Binolfi Andres Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut Liokatis Stamatis Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut Verzini Silvia Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut Selenko Philipp Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut Journal of Biomolecular NMR Springer Netherlands 51/4(2011-12-01), 487-495 0925-2738 51:4<487 2011 51 10858 https://doi.org/10.1007/s10858-011-9577-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9577-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Theillet François-Xavier Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut NATIONALLICENCE 700 1- Binolfi Andres Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut NATIONALLICENCE 700 1- Liokatis Stamatis Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut NATIONALLICENCE 700 1- Verzini Silvia Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut NATIONALLICENCE 700 1- Selenko Philipp Department of NMR-assisted Structural Biology, In-cell NMR Group, Leibniz Institute of Molecular Pharmacology (FMP), Robert-Roessle Str. 10, 13125, Berlin, Germany aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/4(2011-12-01), 487-495 0925-2738 51:4<487 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer