caa a22 4500 445325453 CHVBK 20180317142719.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10858-011-9553-x doi (NATIONALLICENCE)springer-10.1007/s10858-011-9553-x Simultaneous measurement of 1H-15N and Methyl 1Hm-13Cm residual dipolar couplings in large proteins [Elektronische Daten] [Xinli Liao, Raquel Godoy-Ruiz, Chenyun Guo, Vitali Tugarinov] A two-dimensional TROSY-based SIM-13Cm-1Hm/1H-15N NMR experiment for simultaneous measurements of methyl 1 D CH and backbone amide 1 D NH residual dipolar couplings (RDC) in {U-[15N,2H]; Ileδ1-[13CH3]; Leu,Val-[13CH3/12CD3]}-labeled samples of large proteins is described. Significant variation in the alignment tensor of the 82-kDa enzyme Malate synthase G is observed as a function of only slight changes in experimental conditions. The SIM-13Cm-1Hm/1H-15N data sets provide convenient means of establishing the alignment tensor characteristics via the measurement of 1 D NH RDCs in the same protein sample. Springer Science+Business Media B.V., 2011 Transverse relaxation optimized spectroscopy (TROSY) nationallicence Residual dipolar coupling (RDC) nationallicence Alignment tensor nationallicence Malate synthase G (MSG) nationallicence Liao Xinli Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA aut Godoy-Ruiz Raquel Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA aut Guo Chenyun Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA aut Tugarinov Vitali Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA aut Journal of Biomolecular NMR Springer Netherlands 51/1-2(2011-09-01), 191-198 0925-2738 51:1-2<191 2011 51 10858 https://doi.org/10.1007/s10858-011-9553-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9553-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liao Xinli Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA aut NATIONALLICENCE 700 1- Godoy-Ruiz Raquel Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA aut NATIONALLICENCE 700 1- Guo Chenyun Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA aut NATIONALLICENCE 700 1- Tugarinov Vitali Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/1-2(2011-09-01), 191-198 0925-2738 51:1-2<191 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer