caa a22 4500 445325534 CHVBK 20180317142719.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10858-011-9547-8 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9547-8 Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy [Elektronische Daten] [Karin Kloiber, Romana Spitzer, Sarina Grutsch, Christoph Kreutz, Martin Tollinger] Longitudinal exchange experiments facilitate the quantification of the rates of interconversion between the exchanging species, along with their longitudinal relaxation rates, by analyzing the time-dependence of direct correlation and exchange cross peaks. Here we present a simple and robust alternative to this strategy, which is based on the combination of two complementary experiments, one with and one without resolving exchange cross peaks. We show that by combining the two data sets systematic errors that are caused by differential line-broadening of the exchanging species are avoided and reliable quantification of kinetic and relaxation parameters in the presence of additional conformational exchange on the ms-μs time scale is possible. The strategy is applied to a bistable DNA oligomer that displays different line-broadening in the two exchanging species. The Author(s), 2011 Longitudinal exchange nationallicence DNA nationallicence Dynamics nationallicence Line-broadening nationallicence Relaxation nationallicence Kloiber Karin Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut Spitzer Romana Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut Grutsch Sarina Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut Kreutz Christoph Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut Tollinger Martin Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut Journal of Biomolecular NMR Springer Netherlands 51/1-2(2011-09-01), 123-129 0925-2738 51:1-2<123 2011 51 10858 https://doi.org/10.1007/s10858-011-9547-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9547-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kloiber Karin Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Spitzer Romana Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Grutsch Sarina Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Kreutz Christoph Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Tollinger Martin Institute of Organic Chemistry, University of Innsbruck, Innrain 52a, 6020, Innsbruck, Austria aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/1-2(2011-09-01), 123-129 0925-2738 51:1-2<123 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer