caa a22 4500 445325569 CHVBK 20180317142719.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10858-011-9552-y doi (NATIONALLICENCE)springer-10.1007/s10858-011-9552-y Zn-binding AZUL domain of human ubiquitin protein ligase Ube3A [Elektronische Daten] [Alexander Lemak, Adelinda Yee, Irina Bezsonova, Sirano Dhe-Paganon, Cheryl Arrowsmith] Ube3A (also referred to as E6AP for E6 Associated Protein) is a E3 ubiquitin-protein ligase implicated in the development of Angelman syndrome by controlling degradation of synaptic protein Arc and oncogenic papilloma virus infection by controlling degradation of p53. This article describe the solution NMR structure of the conserved N-terminal domain of human Ube3A (residues 24-87) that contains two residues (Cys44 and Arg62) found to be mutated in patients with Angelman syndrome. The structure of this domain adopts a novel Zn-binding fold we called AZUL (Amino-terminal Zn-finger of Ube3a Ligase). The AZUL domain has a helix-loop-helix architecture with a Zn ion coordinated by four Cys residues arranged in Cys-X4-Cys-X4-Cys-X28-Cys motif. Three of the Zn-bound residues are located in a 23-residue long and well structured loop that connects two α-helicies. Springer Science+Business Media B.V., 2011 Ube3A nationallicence E6-AP nationallicence E3A ubiquitin ligase nationallicence AZUL nationallicence Angelman syndrome nationallicence Zn-finger nationallicence HPV nationallicence NMR nationallicence Lemak Alexander Ontario Cancer Institute, Campbell Family Cancer Research Institute and Department of Medical Biophysics, University of Toronto, and Northeast Structural Genomics Consortium, 101 College Street, M5G 1L7, Toronto, ON, Canada aut Yee Adelinda Ontario Cancer Institute, Campbell Family Cancer Research Institute and Department of Medical Biophysics, University of Toronto, and Northeast Structural Genomics Consortium, 101 College Street, M5G 1L7, Toronto, ON, Canada aut Bezsonova Irina Department of Molecular Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, 06030, Farmington, CT, USA aut Dhe-Paganon Sirano Structural Genomics Consortium, University of Toronto, 101 College Street, M5G 1L7, Toronto, ON, Canada aut Arrowsmith Cheryl Ontario Cancer Institute, Campbell Family Cancer Research Institute and Department of Medical Biophysics, University of Toronto, and Northeast Structural Genomics Consortium, 101 College Street, M5G 1L7, Toronto, ON, Canada aut Journal of Biomolecular NMR Springer Netherlands 51/1-2(2011-09-01), 185-190 0925-2738 51:1-2<185 2011 51 10858 https://doi.org/10.1007/s10858-011-9552-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9552-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lemak Alexander Ontario Cancer Institute, Campbell Family Cancer Research Institute and Department of Medical Biophysics, University of Toronto, and Northeast Structural Genomics Consortium, 101 College Street, M5G 1L7, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Yee Adelinda Ontario Cancer Institute, Campbell Family Cancer Research Institute and Department of Medical Biophysics, University of Toronto, and Northeast Structural Genomics Consortium, 101 College Street, M5G 1L7, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Bezsonova Irina Department of Molecular Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, 06030, Farmington, CT, USA aut NATIONALLICENCE 700 1- Dhe-Paganon Sirano Structural Genomics Consortium, University of Toronto, 101 College Street, M5G 1L7, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Arrowsmith Cheryl Ontario Cancer Institute, Campbell Family Cancer Research Institute and Department of Medical Biophysics, University of Toronto, and Northeast Structural Genomics Consortium, 101 College Street, M5G 1L7, Toronto, ON, Canada aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 51/1-2(2011-09-01), 185-190 0925-2738 51:1-2<185 2011 51 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer