caa a22 4500 445325631 CHVBK 20180317142719.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10858-011-9496-2 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9496-2 5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion [Elektronische Daten] [Jiří Nováček, Anna Zawadzka-Kazimierczuk, Veronika Papoušková, Lukáš Žídek, Hana Šanderová, Libor Krásný, Wiktor Koźmiński, Vladimír Sklenář] Two novel 5D NMR experiments (CACONCACO, NCOCANCO) for backbone assignment of disordered proteins are presented. The pulse sequences exploit relaxation properties of the unstructured proteins and combine the advantages of 13C-direct detection, non-uniform sampling, and longitudinal relaxation optimization to maximize the achievable resolution and minimize the experimental time. The pulse sequences were successfully tested on the sample of partially disordered delta subunit from RNA polymerase from Bacillus subtilis. The unstructured part of this 20kDa protein consists of 81 amino acids with frequent sequential repeats. A collection of 0.0003% of the data needed for a conventional experiment with linear sampling was sufficient to perform an unambiguous assignment of the disordered part of the protein from a single 5D spectrum. Springer Science+Business Media B.V., 2011 Intrinsically disordered proteins nationallicence Non-uniform sampling nationallicence 13C detection nationallicence Longitudinal relaxation optimization nationallicence Backbone assignment nationallicence Nováček Jiří Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut Zawadzka-Kazimierczuk Anna Faculty of Chemistry, University of Warsaw, Pasteura 1, 02 093, Warsaw, Poland aut Papoušková Veronika Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut Žídek Lukáš Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut Šanderová Hana Laboratory of Molecular Genetics of Bacteria and Department of Bacteriology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic aut Krásný Libor Laboratory of Molecular Genetics of Bacteria and Department of Bacteriology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic aut Koźmiński Wiktor Faculty of Chemistry, University of Warsaw, Pasteura 1, 02 093, Warsaw, Poland aut Sklenář Vladimír Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut Journal of Biomolecular NMR Springer Netherlands 50/1(2011-05-01), 1-11 0925-2738 50:1<1 2011 50 10858 https://doi.org/10.1007/s10858-011-9496-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9496-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nováček Jiří Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Zawadzka-Kazimierczuk Anna Faculty of Chemistry, University of Warsaw, Pasteura 1, 02 093, Warsaw, Poland aut NATIONALLICENCE 700 1- Papoušková Veronika Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Žídek Lukáš Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Šanderová Hana Laboratory of Molecular Genetics of Bacteria and Department of Bacteriology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic aut NATIONALLICENCE 700 1- Krásný Libor Laboratory of Molecular Genetics of Bacteria and Department of Bacteriology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic aut NATIONALLICENCE 700 1- Koźmiński Wiktor Faculty of Chemistry, University of Warsaw, Pasteura 1, 02 093, Warsaw, Poland aut NATIONALLICENCE 700 1- Sklenář Vladimír Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 50/1(2011-05-01), 1-11 0925-2738 50:1<1 2011 50 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer