caa a22 4500 445325755 CHVBK 20180317142720.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10858-011-9515-3 doi (NATIONALLICENCE)springer-10.1007/s10858-011-9515-3 31P NMR correlation maps of 18O/16O chemical shift isotopic effects for phosphometabolite labeling studies [Elektronische Daten] [Nenad Juranić, Emirhan Nemutlu, Song Zhang, Petras Dzeja, Andre Terzic, Slobodan Macura] Intramolecular correlations among the 18O-labels of metabolic oligophosphates, mapped by J-decoupled 31P NMR 2D chemical shift correlation spectroscopy, impart stringent constraints to the 18O-isotope distributions over the whole oligophosphate moiety. The multiple deduced correlations of isotopic labels enable determination of site-specific fractional isotope enrichments and unravel the isotopologue statistics. This approach ensures accurate determination of 18O-labeling rates of phosphometabolites, critical in biochemical energy conversion and metabolic flux transmission. The biological usefulness of the J-decoupled 31P NMR 2D chemical shift correlation maps was validated on adenosine tri-phosphate fractionally 18O labeled in perfused mammalian hearts. Springer Science+Business Media B.V., 2011 31P NMR chemical shift correlation nationallicence 18O/16O isotopic effects nationallicence ATP turnover nationallicence Isotopologue distribution nationallicence Oxygen-18 nationallicence High-energy phosphoryl dynamics nationallicence Phosphotransfer nationallicence Phosphometabolomics nationallicence Juranić Nenad Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, 55905, Rochester, MN, USA aut Nemutlu Emirhan Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, 55905, Rochester, MN, USA aut Zhang Song Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, 55905, Rochester, MN, USA aut Dzeja Petras Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, 55905, Rochester, MN, USA aut Terzic Andre Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, 55905, Rochester, MN, USA aut Macura Slobodan Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, 55905, Rochester, MN, USA aut Journal of Biomolecular NMR Springer Netherlands 50/3(2011-07-01), 237-245 0925-2738 50:3<237 2011 50 10858 https://doi.org/10.1007/s10858-011-9515-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10858-011-9515-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Juranić Nenad Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, 55905, Rochester, MN, USA aut NATIONALLICENCE 700 1- Nemutlu Emirhan Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, 55905, Rochester, MN, USA aut NATIONALLICENCE 700 1- Zhang Song Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, 55905, Rochester, MN, USA aut NATIONALLICENCE 700 1- Dzeja Petras Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, 55905, Rochester, MN, USA aut NATIONALLICENCE 700 1- Terzic Andre Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, 55905, Rochester, MN, USA aut NATIONALLICENCE 700 1- Macura Slobodan Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, 55905, Rochester, MN, USA aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Springer Netherlands 50/3(2011-07-01), 237-245 0925-2738 50:3<237 2011 50 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer