caa a22 4500 445326352 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10989-011-9240-5 doi (NATIONALLICENCE)springer-10.1007/s10989-011-9240-5 Effects of a Synthetic bFGF Antagonist Peptide on the Proteome of 3T3 Cells Stimulated with bFGF [Elektronische Daten] [Xiaoping Wu, Xinglong Jia, Yuhua Ji, Cong Wang, Yonglin Yu, Susu Gao, Quchou Li, Xiaokun Li] It has been known that basic fibroblast growth factor (bFGF) plays an important role in tumor progression mainly due to its strong mitogenic activity. Antagonists targeting bFGF have been considered a potential strategy for cancer therapy via inhibiting cell proliferation induced by bFGF. We have previously obtained a high-affinity bFGF-binding peptide (named as P7) with strong inhibitory activity against bFGF-induced cell proliferation from the phage display random heptapeptide library. The aim of the present investigation was to synthesize the peptide P7 by solid phase method and explore the mechanisms of the inhibitory effect of P7-targeting bFGF stimulation on Balb/c 3T3 cells via proteomic analysis. Seven differentially expressed protein were identified, among which four were decreased by bFGF stimulation alone and increased by addition of P7, the other three were up-regulated by bFGF treatment alone and down-regulated by addition of P7. Among the identified proteins, epidermal fatty acid-binding protein (E-FABP) and low molecular weight phosphotyrosine protein phosphatase (LMW-PTP) take part in the regulation of cell proliferation. The results suggested P7 inhibited the bioactivities of bFGF possibly via influencing the expression of cellular proteins related to cell proliferation. Springer Science+Business Media, LLC, 2011 BFGF antagonist peptide nationallicence Solid phase nationallicence Proteome nationallicence Proliferation nationallicence Epidermal fatty acid-binding protein nationallicence Wu Xiaoping Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut Jia Xinglong Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut Ji Yuhua Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut Wang Cong Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut Yu Yonglin Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut Gao Susu Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut Li Quchou Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut Li Xiaokun School of Pharmaceutical Science, Wenzhou Medical College, 325035, Wenzhou, China aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/1(2011-03-01), 53-59 1573-3149 17:1<53 2011 17 10989 https://doi.org/10.1007/s10989-011-9240-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9240-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Xiaoping Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Jia Xinglong Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Ji Yuhua Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Cong Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Yu Yonglin Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Gao Susu Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Li Quchou Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Li Xiaokun School of Pharmaceutical Science, Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/1(2011-03-01), 53-59 1573-3149 17:1<53 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer