caa a22 4500 445326379 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10989-011-9242-3 doi (NATIONALLICENCE)springer-10.1007/s10989-011-9242-3 Mimotopes to Cetuximab Isolated by mRNA-Display using Random Peptide Libraries and their Characteristics [Elektronische Daten] [Tatsuro Shibui, Teruaki Kobayashi] To obtain epitope mimic peptides, mimotopes, for vaccine development, mRNA display technology was applied to Cetuximab. After six cycles of affinity selection, four clones that bound to Cetuximab were isolated that showed specific binding to the antibody in pull-down assays. Their random peptide portions were chemically synthesized and conjugated with keyhole limpet hemocyanin (KLH). Their ability to elicit production of antibodies that bind to the antibody's original antigen, the human EGF receptor, was examined by vaccination in rabbits five times bi-weekly. Although the peptides and their KLH conjugates showed different characteristics in vitro and further analysis is needed, the results of immunizations showed that the mimotope peptides isolated from mRNA display libraries could generate antibodies against the antibody's original antigen in vivo. Springer Science+Business Media, LLC, 2011 Random peptide nationallicence mRNA display nationallicence Epitope nationallicence Minotope nationallicence Antigen nationallicence Antibody nationallicence Vaccine nationallicence Shibui Tatsuro MOLECUENCE Corporation, Mitsubishi Chemical Group Yokohama Research Center, 1000 Kamoshida-cho, Aoba-ku, 227-8502, Yokohama, Kanagawa, Japan aut Kobayashi Teruaki MOLECUENCE Corporation, Mitsubishi Chemical Group Yokohama Research Center, 1000 Kamoshida-cho, Aoba-ku, 227-8502, Yokohama, Kanagawa, Japan aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/1(2011-03-01), 69-74 1573-3149 17:1<69 2011 17 10989 https://doi.org/10.1007/s10989-011-9242-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9242-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shibui Tatsuro MOLECUENCE Corporation, Mitsubishi Chemical Group Yokohama Research Center, 1000 Kamoshida-cho, Aoba-ku, 227-8502, Yokohama, Kanagawa, Japan aut NATIONALLICENCE 700 1- Kobayashi Teruaki MOLECUENCE Corporation, Mitsubishi Chemical Group Yokohama Research Center, 1000 Kamoshida-cho, Aoba-ku, 227-8502, Yokohama, Kanagawa, Japan aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/1(2011-03-01), 69-74 1573-3149 17:1<69 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer