caa a22 4500 445326387 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10989-011-9255-y doi (NATIONALLICENCE)springer-10.1007/s10989-011-9255-y Antibacterial Activity, Cytotoxicity and Mechanisms of action of Cathelicidin Peptides against Enteric Pathogens in Weaning Piglets [Elektronische Daten] [YiFan Liu, Chao Luan, Xi Xia, Sha An, YiZhen Wang] In the last few decades, long-term and high-dose usage of antibiotics in livestock diets has led to the emergence of antibiotic resistant bacteria, antibiotic residues in animal products and environmental pollution, adversely affecting animal health. Because of these concerns, a study screening cathelicidin peptides from different animal origins (i.e. protegrin-1 [PG-1], PMAP-23, LL-37, indolicidin and cathelicidin-BF [C-BF]) as antibiotic replacements with higher antimicrobial activity and lower cytotoxicity was designed to study their mechanisms towards enteric pathogens in weaning piglets. PG-1 and C-BF proved to be the most effective bacteriocids with the widest spectra of activity, with the MIC values equal to or lower than commonly used antibiotics towards several Escherichia and Salmonella strains, and showed a synergistic effect with aureomycin. Mechanism studies suggested the C-BF killing mechanism is based on membrane permeability, while multiple targets maybe exist for PG-1, including membrane and intracellular biomacromolecules. Cytotoxicity tests showed PMAP-23 and C-BF exhibited the lowest cytotoxic effects, while PG-1, LL-37 and indolicidin displayed cytotoxicity by dose. This study demonstrated that among the peptides tested, C-BF has the capacity to inactivate enteric pathogens with lower cytotoxicity and is potentially a novel anti-bacterial agent. The activity of PG-1 is highly efficient, with the potential to reduce cytotoxicity using molecular design. Springer Science+Business Media, LLC, 2011 Cathelicidin peptides nationallicence Antibacterial activity nationallicence Cytotoxicity nationallicence Mechanisms of action nationallicence Pathogenic enterobacteria nationallicence Weaning piglets nationallicence Liu YiFan National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut Luan Chao National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut Xia Xi National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut An Sha National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut Wang YiZhen National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 175-184 1573-3149 17:3<175 2011 17 10989 https://doi.org/10.1007/s10989-011-9255-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9255-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu YiFan National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Luan Chao National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Xia Xi National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- An Sha National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Wang YiZhen National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control; Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Institute of Feed Science, Zhejiang University, 310029, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 175-184 1573-3149 17:3<175 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer