caa a22 4500 445326395 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10989-011-9259-7 doi (NATIONALLICENCE)springer-10.1007/s10989-011-9259-7 Comparison of Methods for Chemical Conjugation of an Influenza Peptide to Wild-Type and Cysteine-Mutant Virus-Like Particles Expressed in Pseudomonas fluorescens [Elektronische Daten] [Greg Cantin, Sol Resnick, Hongfan Jin, Ryan O'Hanlon, Octavio Espinosa, Alex Stevens, Jason Payne, Nicole Glenn, Lada Rasochova, Jeffrey Allen] Chemical conjugation of the influenza peptide antigen M2E to different variants of virus-like particles (VLPs) was investigated. Wild-type cowpea chlorotic mottle virus (CCMV) and two novel cysteine mutants of CCMV, all expressed in Pseudomonas fluorescens, were utilized in this study. Two different conjugation schemes, primary amine-directed and cysteine-directed, were tested and compared. Both strategies were successfully used to attach M2E peptides to the surface of these VLPs. Ultimately, the cysteine-directed conjugation strategy using the CCMV cysteine mutant particles displayed key advantages over the primary amine-directed strategy. Springer Science+Business Media, LLC, 2011 Virus-like particle (VLP) nationallicence Cowpea chlorotic mottle virus (CCMV) nationallicence Influenza peptide conjugation nationallicence Conjugate vaccine nationallicence Pseudomonas fluorescens nationallicence Recombinant VLP Production nationallicence Cantin Greg Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut Resnick Sol Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut Jin Hongfan Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut O'Hanlon Ryan Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut Espinosa Octavio Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut Stevens Alex Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut Payne Jason Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut Glenn Nicole Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut Rasochova Lada Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut Allen Jeffrey Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 217-224 1573-3149 17:3<217 2011 17 10989 https://doi.org/10.1007/s10989-011-9259-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9259-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cantin Greg Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Resnick Sol Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Jin Hongfan Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- O'Hanlon Ryan Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Espinosa Octavio Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Stevens Alex Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Payne Jason Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Glenn Nicole Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Rasochova Lada Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Allen Jeffrey Pfenex Inc., 10790 Roselle Street, 92121, San Diego, CA, USA aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 217-224 1573-3149 17:3<217 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer