caa a22 4500 445326425 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10989-011-9260-1 doi (NATIONALLICENCE)springer-10.1007/s10989-011-9260-1 Radical Scavenging Activity of Seela ( Sphyraena barracuda) and Ribbon Fish ( Lepturacanthus savala) Backbone Protein Hydrolysates [Elektronische Daten] [R. Nazeer, R. Deeptha, R. Jaiganesh, N. Sampathkumar, Shabeena Naqash] We have investigated the antioxidant activity of protein hydrolysates prepared from backbones of two commercially important fishes; seela (Sphyraena barracuda) and ribbon fish (Lepturacanthus savala). Pepsin and trypsin hydrolysates were found more potent to inhibit lipid peroxidation in case of ribbon and seela fish respectively and were further purified by using fast protein liquid chromatography on anion exchange and gel filtration chromatography. The active peaks after gel filtration chromatography of seela fish was able to scavenge 2,2-diphenyl-1-picryhydrazyl and hydroxyl radicals by 61±2.3 and 58.7±2.3% and ribbon fish hydrolysate by 60.0±2.6 and 55.6±1.8% as measured by ESR spectroscopy. And the active fractions showed presence of both essential and non-essential amino acids with high percentages of arginine (11.95 and 12.76%) and lysine (13.49 and 13.89%). Springer Science+Business Media, LLC, 2011 Antioxidants nationallicence ESR spectroscopy nationallicence Sphyraena barracuda nationallicence Lepturacanthus savala nationallicence Lipid peroxidation nationallicence Nazeer R. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut Deeptha R. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut Jaiganesh R. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut Sampathkumar N. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut Naqash Shabeena Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 209-216 1573-3149 17:3<209 2011 17 10989 https://doi.org/10.1007/s10989-011-9260-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9260-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nazeer R. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut NATIONALLICENCE 700 1- Deeptha R. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut NATIONALLICENCE 700 1- Jaiganesh R. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut NATIONALLICENCE 700 1- Sampathkumar N. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut NATIONALLICENCE 700 1- Naqash Shabeena Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 209-216 1573-3149 17:3<209 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer