caa a22 4500 445326441 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10989-011-9254-z doi (NATIONALLICENCE)springer-10.1007/s10989-011-9254-z Isolation and Characterization of a Natriuretic Peptide from Crotalus oreganus abyssus (Grand Canyon Rattlesnake) and its Effects on Systemic Blood Pressure and Nitrite Levels [Elektronische Daten] [S. Da Silva, J. Almeida, L. Resende, W. Martins, F. Henriques, P. Baldasso, A. Soares, A. Taranto, R. Resende, S. Marangoni, C. Dias-Junior] Studies on the therapeutic potential of venom peptides have significantly advanced the development of new peptide drugs. A good example is captopril, a synthetic peptide drug, which acts as an anti-hypertensive and potentiating bradykinin, inhibiting the angiotensin-converting enzyme, whose precursor was isolated from the venom of Bothrops jararacussu. The natriuretic peptide (NPs) family comprises three members, ANP (atrial natriuretic peptide), BNP (B-type natriuretic peptide) and CNP (C-type natriuretic peptide), and has an important role in blood pressure regulation and electrolyte homeostasis. In this study, we describe, for the first time, the isolation and characterization of a novel natriuretic-like peptide (Coa_NP), isolated from Crotalus Oreganus abyssus venom. The peptide has 32 amino acids and its complete sequence is SKRLSNGCFGLKLDRIGAMSGLGCWRLINESK. The Coa_NP has an average molecular mass of 3510.98Da and its amino acid sequence presents the loop region that is characteristic of natriuretic peptides (17 amino acids, NP domain consensus; CFGXXXDRIXXXSGLGC). Coa_NP is a natriuretic peptide of the ANP/BNP-like family, since the carboxy terminal region of CNP has its own NP domain. The functional experiments showed that Coa_NP produced biological effects similar to those of the other natriuretic peptides: (1) a dose-dependent decrease in mean arterial pressure; (2) significant increases in plasma nitrite levels, and (3) vasorelaxation in thoracic aortic rings that were pre-contracted with phenylephrine. The structural and biological aspects confirm Coa_NP as a natriuretic peptide isolated from snake venom, thus expanding the diversification of venom components. Springer Science+Business Media, LLC, 2011 Natriuretic peptide nationallicence Crotalus oreganus abyssus nationallicence Bioactive peptides nationallicence Nitric oxide nationallicence Da Silva S. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut Almeida J. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut Resende L. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut Martins W. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut Henriques F. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut Baldasso P. Department of Biochemistry, Institute of Biology, State University of Campinas, Campinas, SP, Brazil aut Soares A. Department of Toxicology, Faculty of Phamaceutics Sciences, São Paulo University, Ribeirão Preto, SP, Brazil aut Taranto A. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut Resende R. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut Marangoni S. Department of Biochemistry, Institute of Biology, State University of Campinas, Campinas, SP, Brazil aut Dias-Junior C. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 165-173 1573-3149 17:3<165 2011 17 10989 https://doi.org/10.1007/s10989-011-9254-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9254-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Da Silva S. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut NATIONALLICENCE 700 1- Almeida J. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut NATIONALLICENCE 700 1- Resende L. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut NATIONALLICENCE 700 1- Martins W. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut NATIONALLICENCE 700 1- Henriques F. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut NATIONALLICENCE 700 1- Baldasso P. Department of Biochemistry, Institute of Biology, State University of Campinas, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Soares A. Department of Toxicology, Faculty of Phamaceutics Sciences, São Paulo University, Ribeirão Preto, SP, Brazil aut NATIONALLICENCE 700 1- Taranto A. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut NATIONALLICENCE 700 1- Resende R. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut NATIONALLICENCE 700 1- Marangoni S. Department of Biochemistry, Institute of Biology, State University of Campinas, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Dias-Junior C. Federal University of São João Del Rei, Campus Centro-oeste, Rua Sebastião Gonçalves Coelho 400, 35501-296, Divinopolis, MG, Brazil aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 165-173 1573-3149 17:3<165 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer