caa a22 4500 445326468 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10989-011-9261-0 doi (NATIONALLICENCE)springer-10.1007/s10989-011-9261-0 Effect of a Pool of Peptides Isolated from Crotalus durissus terrificus (South American Rattlesnake) Venom on Glucose Levels of Mice Fed on a High-Fat Diet [Elektronische Daten] [M. Martins-Santos, R. Resende, F. Pinto, A. Soares, S. Marangoni, E. Oliveira, F. Albericio, S. Da Silva] This study investigated the effect of a pool of peptides, isolated from venom of Crotalus durissus terrificus (South American rattlesnake) on glucose concentration in C57BL/6 mice fed on a high-fat diet for 6weeks. The pool of peptides (molecular mass around of 10kDa) was obtained using a MidJet apparatus with a cartridge of 10KDa. The peptide pool was injected intraperitoneally in mice in a single dose (0.5mg/animal) or multiple doses (0.2mg/dose). After predetermined times (30, 60, 90 and 120min) post injections, venous blood samples were collected for enzymatic measurement of serum glucose using a commercial glucose kit (glucose oxidase method). High-fat fed mice showed an increase in blood glucose concentration, in comparison with mice fed on the chow diet. Thirty minutes after a single dose of the peptide pool, high-fat fed animals showed a significant decrease (~47%) in glycemia. However, the glucose level increased again at 60 and 120min. Conversely, after multiple injections of the pool of peptides administered every 30min, the blood glucose concentration in the high-fat mice was significantly decreased (~37%) and remained at low levels until 120min. These results suggest that the tested pool of peptides from Crotalus durissus terrificus contained a peptide (or peptides) with a beneficial role on glucose-lowering action of high-fat fed mice. Springer Science+Business Media, LLC, 2011 Bioactive peptides nationallicence Crotalus durissus terrificus nationallicence Snake venom nationallicence Hyperglycemia nationallicence Glucose nationallicence T2DM : Diabetes mellitus type 2 nationallicence PPAR- γ : Peroxisome proliferator-activated receptor-γ nationallicence GLP : Glucagon-like peptide analogues nationallicence DPP-4 : Dipeptidyl peptidase-4 nationallicence HF : Mice treated with high fat diet nationallicence HFS : Mice treated with high fat diet and injected with saline nationallicence HFT : Mice treated with high fat diet and injected with a pool of peptide nationallicence PP : Pool of peptide nationallicence Martins-Santos M. Midwest Campus (CCO), Federal University of São João Del Rei (FUSJ), Rua Sebastião Gonçalves Coelho, 400, 35501-296, Divinópolis, MG, Brazil aut Resende R. Midwest Campus (CCO), Federal University of São João Del Rei (FUSJ), Rua Sebastião Gonçalves Coelho, 400, 35501-296, Divinópolis, MG, Brazil aut Pinto F. Midwest Campus (CCO), Federal University of São João Del Rei (FUSJ), Rua Sebastião Gonçalves Coelho, 400, 35501-296, Divinópolis, MG, Brazil aut Soares A. Department of Clinical Analysis of the Faculty of Pharmaceutical Sciences of Ribeirão Preto, São Paulo University, 14040-903, Ribeirão Preto, SP, Brazil aut Marangoni S. Department of Biochemistry of Biology Institute, State University of Campinas, 13083-970, Campinas, SP, Brazil aut Oliveira E. Proteomics Platform, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028, Barcelona, Spain aut Albericio F. Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028, Barcelona, Spain aut Da Silva S. Midwest Campus (CCO), Federal University of São João Del Rei (FUSJ), Rua Sebastião Gonçalves Coelho, 400, 35501-296, Divinópolis, MG, Brazil aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 225-230 1573-3149 17:3<225 2011 17 10989 https://doi.org/10.1007/s10989-011-9261-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9261-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martins-Santos M. Midwest Campus (CCO), Federal University of São João Del Rei (FUSJ), Rua Sebastião Gonçalves Coelho, 400, 35501-296, Divinópolis, MG, Brazil aut NATIONALLICENCE 700 1- Resende R. Midwest Campus (CCO), Federal University of São João Del Rei (FUSJ), Rua Sebastião Gonçalves Coelho, 400, 35501-296, Divinópolis, MG, Brazil aut NATIONALLICENCE 700 1- Pinto F. Midwest Campus (CCO), Federal University of São João Del Rei (FUSJ), Rua Sebastião Gonçalves Coelho, 400, 35501-296, Divinópolis, MG, Brazil aut NATIONALLICENCE 700 1- Soares A. Department of Clinical Analysis of the Faculty of Pharmaceutical Sciences of Ribeirão Preto, São Paulo University, 14040-903, Ribeirão Preto, SP, Brazil aut NATIONALLICENCE 700 1- Marangoni S. Department of Biochemistry of Biology Institute, State University of Campinas, 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Oliveira E. Proteomics Platform, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Albericio F. Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Da Silva S. Midwest Campus (CCO), Federal University of São João Del Rei (FUSJ), Rua Sebastião Gonçalves Coelho, 400, 35501-296, Divinópolis, MG, Brazil aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 225-230 1573-3149 17:3<225 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer