caa a22 4500 445326476 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10989-011-9262-z doi (NATIONALLICENCE)springer-10.1007/s10989-011-9262-z Antioxidant Peptides from the Protein Hydrolysates of Conus betulinus [Elektronische Daten] [R. Nazeer, T. Srividhya] The current study aims at the isolation and characterization of the peptides, believed to have antioxidant activity, from Conus betulinus by using different types of enzymes. The body and viscera of C. betulinus were treated with three enzymes viz. trypsin, pepsin and papain to obtain peptide hydrolysates. The activities of the hydrolysates were analyzed by DPPH and hydroxyl radical assay by using electron spin resonance (ESR) device. Active hydrolysates were purified using ion exchange chromatography followed by gel filtration chromatography. The activity of the separated fractions was analyzed by ESR; in which the result showed that trypsin hydrolysate of body (28.48 and 76.00%) and viscera (38.45 and 83.00%) respectively have high activity than the other hydrolysates. The HPLC result of purified fraction showed, presence of active amino acids viz., metheonine, cystine, histidine etc. This purified peptide has more antioxidant activity that could reduce the excess free radicals in body in order to prevent free radical induced diseases. Springer Science+Business Media, LLC, 2011 Conus betulinus nationallicence Antioxidant peptides nationallicence Protein hydrolysates nationallicence DPPH scavenging activity nationallicence FPLC nationallicence Nazeer R. Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulatur, Tamilnadu, India aut Srividhya T. Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulatur, Tamilnadu, India aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 231-237 1573-3149 17:3<231 2011 17 10989 https://doi.org/10.1007/s10989-011-9262-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9262-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nazeer R. Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulatur, Tamilnadu, India aut NATIONALLICENCE 700 1- Srividhya T. Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulatur, Tamilnadu, India aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/3(2011-09-01), 231-237 1573-3149 17:3<231 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer