caa a22 4500 445326557 CHVBK 20180317142722.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10989-011-9270-z doi (NATIONALLICENCE)springer-10.1007/s10989-011-9270-z Purification of Lectin from Micropropagated Roots Derived from Aseptic Seedling of Canavalia ensiformis L [Elektronische Daten] [Samreen Amani, Afshin Iram, Anwar Shahzad, Km Neelofar, Aabgeena Naeem] A well-organized protocol has been developed for high frequency root germination from the seed of Canavalia ensiformis on Murashige and Skoog (MS) medium. Surprisingly, the seeds that were grown on the MS medium having no growth hormone showed the best response. Roots of 30days old aseptic seedling were homogenized and a lectin from them was purified on Sephadex G-50 affinity column. The finding that final product is a pure lectin was confirmed by specific hemagglutinating property. The final root lectin yield was 0.6% and eluted as a single peak. Root lectin specific activity was 50 times more than the seed lectin. Sugar specificity activity by hemagglutination-inhibition assay indicated that lectin belongs to glucose/mannose-specific group. Interestingly, the lectin was found to be 25kDa, similar to molecular mass of Concanavalin A purified from seed of C. ensiformis, as revealed by SDS-PAGE. Thus, Concanavalin A from either source can be used for development of transgenic crops that are capable of expressing lectin gene and hence can efficiently perform biological nitrogen fixation by giving rise to nodules in their root. The advantage of this method is that purification of Concanavalin A in tissue culture conditions is easier, handy and is less time consuming. Springer Science+Business Media, LLC, 2011 Affinity chromatography nationallicence C anavalia ensiformis nationallicence Hemagglutination nationallicence Lectin nationallicence MS medium nationallicence Amani Samreen Department of Biochemistry, Faculty of Life Science, AMU, 202 002, Aligarh, India aut Iram Afshin Department of Biochemistry, Faculty of Life Science, AMU, 202 002, Aligarh, India aut Shahzad Anwar Department of Botany, Faculty of Life Science, AMU, 202 002, Aligarh, India aut Neelofar Km Department of Botany, Faculty of Life Science, AMU, 202 002, Aligarh, India aut Naeem Aabgeena Department of Biochemistry, Faculty of Life Science, AMU, 202 002, Aligarh, India aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/4(2011-12-01), 317-324 1573-3149 17:4<317 2011 17 10989 https://doi.org/10.1007/s10989-011-9270-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9270-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Amani Samreen Department of Biochemistry, Faculty of Life Science, AMU, 202 002, Aligarh, India aut NATIONALLICENCE 700 1- Iram Afshin Department of Biochemistry, Faculty of Life Science, AMU, 202 002, Aligarh, India aut NATIONALLICENCE 700 1- Shahzad Anwar Department of Botany, Faculty of Life Science, AMU, 202 002, Aligarh, India aut NATIONALLICENCE 700 1- Neelofar Km Department of Botany, Faculty of Life Science, AMU, 202 002, Aligarh, India aut NATIONALLICENCE 700 1- Naeem Aabgeena Department of Biochemistry, Faculty of Life Science, AMU, 202 002, Aligarh, India aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/4(2011-12-01), 317-324 1573-3149 17:4<317 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer