caa a22 4500 44532659X CHVBK 20180317142723.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10989-011-9250-3 doi (NATIONALLICENCE)springer-10.1007/s10989-011-9250-3 Spontaneous Cleavage of Proteins at Serine Residues [Elektronische Daten] [Brian Lyons, Joanne Jamie, Roger Truscott] Long-lived proteins are found at several sites in the body and they undergo numerous changes as a result of prolonged exposure to physiological conditions. Truncation is a common modification and many cleavages appear to be non-enzymatic, however little is known about the processes involved. In this study we demonstrate, using synthetic peptides that incorporate the sequence of a protein that is known to cleave in older lenses, that truncation on the N-terminal side of serine residues can occur at neutral pH. A mechanism that incorporates an N,O-acyl shift, which is analogous to intein cleavage, is proposed. Such cleavages may explain the origin of abundant peptides derived from crystallins in aged human lenses. Springer Science+Business Media, LLC, 2011 Old proteins nationallicence Age nationallicence Hydrolysis nationallicence Posttranslational modification nationallicence Human lens nationallicence Lyons Brian Save Sight Institute, University of Sydney, Sydney Eye Hospital, 8 Macquarie St., 2001, Sydney, NSW, Australia aut Jamie Joanne Department of Chemistry and Biomolecular Sciences, Macquarie University, 2109, Sydney, NSW, Australia aut Truscott Roger Save Sight Institute, University of Sydney, Sydney Eye Hospital, 8 Macquarie St., 2001, Sydney, NSW, Australia aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/2(2011-06-01), 131-135 1573-3149 17:2<131 2011 17 10989 https://doi.org/10.1007/s10989-011-9250-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9250-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lyons Brian Save Sight Institute, University of Sydney, Sydney Eye Hospital, 8 Macquarie St., 2001, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Jamie Joanne Department of Chemistry and Biomolecular Sciences, Macquarie University, 2109, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Truscott Roger Save Sight Institute, University of Sydney, Sydney Eye Hospital, 8 Macquarie St., 2001, Sydney, NSW, Australia aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/2(2011-06-01), 131-135 1573-3149 17:2<131 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer