caa a22 4500 445326603 CHVBK 20180317142723.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10989-011-9245-0 doi (NATIONALLICENCE)springer-10.1007/s10989-011-9245-0 Fluorescence Studies on a Designed Peptide of REIP as a Potential Hydrophobic Drug Carrier [Elektronische Daten] [Yongzhi Lu, Xiaojun Zhao] Many hydrophobic drugs have limited or no activity in clinical application due to their poor aqueous solubility. In order to find fine delivery system, we designed a novel peptide REIP (Ac-RIEIRIEIAPAIEIRIEIR) and investigated its properties as a delivery system. Microcrystal of pyrene could be stabilized by the peptide REIP and suspended in aqueous solution. We used pyrene as a model hydrophobic drug and egg phosphatidylcholine (EPC) vesicles as model membranes to study the ability of REIP in hydrophobic drug encapsulation and transfer to EPC vesicles (liposome) by analyzing the fluorescence spectroscopy of pyrene. It was found that pyrene was present in the crystalline form when stabilized by REIP and was able to transfer into EPC vesicles in molecular form. The concentration of pyrene released into the EPC vesicles at a given time was quantified using a calibration curve. Double exponential curve fitting was better for the release profiles of REIP-Py solution than single exponential curve fitting. Springer Science+Business Media, LLC, 2011 Peptide nationallicence Pyrene nationallicence Hydrophobic drug carrier nationallicence Fluorescence spectroscopy nationallicence Liposome nationallicence Lu Yongzhi West China Hospital Nanomedicine Laboratory and Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, 610065, Sichuan, China aut Zhao Xiaojun West China Hospital Nanomedicine Laboratory and Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, 610065, Sichuan, China aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/2(2011-06-01), 81-86 1573-3149 17:2<81 2011 17 10989 https://doi.org/10.1007/s10989-011-9245-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9245-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Yongzhi West China Hospital Nanomedicine Laboratory and Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, 610065, Sichuan, China aut NATIONALLICENCE 700 1- Zhao Xiaojun West China Hospital Nanomedicine Laboratory and Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, 610065, Sichuan, China aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/2(2011-06-01), 81-86 1573-3149 17:2<81 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer