caa a22 4500 445326611 CHVBK 20180317142723.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10989-011-9243-2 doi (NATIONALLICENCE)springer-10.1007/s10989-011-9243-2 Glutathione (GSH) Determination by a Very Simple Electrochemical Method [Elektronische Daten] [Mustafa Sezgintürk, Erhan Dinçkaya] Glutathione (GSH; gamma-glutamylcysteinylglycine) is ubiquitous in mammalian and other living cells. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. It has several important functions, including protection against oxidative stress. It is synthesized from its constituent amino acids by the consecutive actions of gamma-glutamylcysteine synthetase and GSH synthetase. Cellular levels of GSH may be increased by supplying substrates and GSH delivery compounds. Increasing cellular GSH may be therapeutically useful. In this study, we investigated the applicability of the glassy carbon electrode coated with thin Hg film layer to the determination of reduced glutathione (GSH). For this purpose, firstly, Hg coating process parameters were studied such as concentration of mercury coating solution, coating current, coating temperature. Then, working conditions were investigated. At the end of these studies, we concluded that although some of limitations, the sensor would be applicable to the determination of reduced glutathione. Springer Science+Business Media, LLC, 2011 Glutathione nationallicence Thin Hg films nationallicence GSH nationallicence Biosensor nationallicence Mercury film electrode nationallicence Sezgintürk Mustafa Chemistry Department, Faculty of Arts and Science, Namık Kemal University, 59100, Tekirdağ, Turkey aut Dinçkaya Erhan Biochemistry Department, Faculty of Science, Ege University, Bornova, 35100, Izmir, Turkey aut International Journal of Peptide Research and Therapeutics Springer Netherlands 17/2(2011-06-01), 87-92 1573-3149 17:2<87 2011 17 10989 https://doi.org/10.1007/s10989-011-9243-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10989-011-9243-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sezgintürk Mustafa Chemistry Department, Faculty of Arts and Science, Namık Kemal University, 59100, Tekirdağ, Turkey aut NATIONALLICENCE 700 1- Dinçkaya Erhan Biochemistry Department, Faculty of Science, Ege University, Bornova, 35100, Izmir, Turkey aut NATIONALLICENCE 773 0- International Journal of Peptide Research and Therapeutics Springer Netherlands 17/2(2011-06-01), 87-92 1573-3149 17:2<87 2011 17 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer