caa a22 4500 445327464 CHVBK 20180317142725.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10953-010-9631-9 doi (NATIONALLICENCE)springer-10.1007/s10953-010-9631-9 Interactions of Hemoglobin with Vesicles and Tubes Formed from Mixtures of Histidine-Derived Bolaamphiphile and Conventional Surfactants [Elektronische Daten] [Yuxia Chen, Yan Liu, Rong Guo, Shuyong Xian] The interactions between protein and surfactant aggregates have been the subject of intensive studies due to their potential applications in biological systems. Here we report the interactions of hemoglobin (Hb) with vesicles and tube-like aggregates formed from mixtures of a histidine-derived bolaamphiphile and a cconventional surfactant dodecyltrimethylammonium bromide (DTAB). This study was performed using a combination of UV-vis spectroscopy, steady and synchronous fluorescence spectroscopy, circular dichroism, and microcalorimetry measurements. The secondary structure of the protein is disturbed, and then the partially unfolded protein is capable of penetrating the vesicles and tube-like aggregates, this is mainly the result of hydrogen bonding and hydrophobic interactions between Hb and the H2D/DTAB aggregates. The polar portion of the unfolded protein chains is near to the polar head of the amphiphile in the aggregate's membrane. Hb is converted to hemichrome in the vesicles, and the heme monomer is solubilized in tube-like aggregates after escaping from the hydrophobic cavity of Hb. Springer Science+Business Media, LLC, 2010 1,12-Dihistidine nationallicence Diaminododecane nationallicence Hemoglobin nationallicence Vesicles nationallicence Tube-like structure nationallicence Interaction nationallicence Chen Yuxia School of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, P.R. China aut Liu Yan School of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, P.R. China aut Guo Rong School of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, P.R. China aut Xian Shuyong School of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, P.R. China aut Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/1(2011-01-01), 48-60 0095-9782 40:1<48 2011 40 10953 https://doi.org/10.1007/s10953-010-9631-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10953-010-9631-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Yuxia School of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, P.R. China aut NATIONALLICENCE 700 1- Liu Yan School of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, P.R. China aut NATIONALLICENCE 700 1- Guo Rong School of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, P.R. China aut NATIONALLICENCE 700 1- Xian Shuyong School of Chemistry and Chemical Engineering, Yangzhou University, 225002, Yangzhou, P.R. China aut NATIONALLICENCE 773 0- Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/1(2011-01-01), 48-60 0095-9782 40:1<48 2011 40 10953 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer