caa a22 4500 445328029 CHVBK 20180317142727.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10953-011-9664-8 doi (NATIONALLICENCE)springer-10.1007/s10953-011-9664-8 Study on the Interaction of Pentachlorophenol withUrease in Aqueous Solution by Multiple Spectroscopic Techniques [Elektronische Daten] [Yan-Qing Wang, Gen-Cheng Zhang, Hong-Mei Zhang] The interactions between pentachlorophenol (PCP) and jack bean urease were studied using UV/vis absorption, CD, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectroscopic techniques. The fluorescence data showed that the fluorescence quenching of urease by PCP the results of the formation of a PCP-urease complex involving a hydrophobic interaction. The distance r between the donor (urease) and acceptor (PCP) was obtained from the fluorescence resonance energy transfer. The effect of PCP on the conformation of urease was analyzed using UV/vis absorption, synchronous fluorescence and three-dimensional fluorescence spectroscopic techniques. The result showed that PCP can enter into the hydrophobic pocket at the interface of urease and that the micro environments around the tyrosine and tryptophan residues were changed. Springer Science+Business Media, LLC, 2011 Fluorescence quenching nationallicence Interaction nationallicence Pentachlorophenol nationallicence Three-dimensional fluorescence spectra nationallicence Urease nationallicence Wang Yan-Qing Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, 224002, Yancheng City, Jiangsu Province, People's Republic of China aut Zhang Gen-Cheng Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, 224002, Yancheng City, Jiangsu Province, People's Republic of China aut Zhang Hong-Mei Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, 224002, Yancheng City, Jiangsu Province, People's Republic of China aut Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/3(2011-03-01), 458-469 0095-9782 40:3<458 2011 40 10953 https://doi.org/10.1007/s10953-011-9664-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10953-011-9664-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Yan-Qing Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, 224002, Yancheng City, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Gen-Cheng Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, 224002, Yancheng City, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hong-Mei Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, 224002, Yancheng City, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/3(2011-03-01), 458-469 0095-9782 40:3<458 2011 40 10953 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer