caa a22 4500 445328088 CHVBK 20180317142728.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10953-011-9653-y doi (NATIONALLICENCE)springer-10.1007/s10953-011-9653-y Study on the Synergism Effect of Lomefloxacin andOfloxacin for Bovine Serum Albumin in Solution bySpectroscopic Techniques [Elektronische Daten] [Xianyong Yu, Ronghua Liu, Danhong Ji, Fengxian Yang, Xiaofang Li, Jian Xie, Jiming Zhou, Pinggui Yi] Both lomefloxacin (LOM) and ofloxacin (OFL) have a powerful ability to quench the fluorescence of bovine serum albumin (BSA). The fluorescence quenching action is much stronger when the two drugs coexist. The synergism between LOM and OFL was studied using fluorescence and ultraviolet spectroscopy under imitated physiological conditions. The results show that static quenching and non-radiation energy transfer are the main reasons for the fluorescence quenching. The synergism results in both the reduction of the binding stability between drugs and BSA and an increase of the free drug concentration, which will increase the efficacy of drugs. The thermodynamic parameters at different temperatures were calculated and the binding distances r between the drugs and BSA were obtained based on Försters theory of non-radiation energy transfer. The synchronous fluorescence spectra indicated that the effect of synergism affected the conformation of BSA. Springer Science+Business Media, LLC, 2011 Fluorescence spectrum nationallicence Bovine serum albumin nationallicence Synergism nationallicence Lomefloxacin nationallicence Ofloxacin nationallicence Yu Xianyong Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut Liu Ronghua Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut Ji Danhong Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut Yang Fengxian Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut Li Xiaofang Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut Xie Jian Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, 92037, La Jolla, CA, USA aut Zhou Jiming Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut Yi Pinggui Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/3(2011-03-01), 521-531 0095-9782 40:3<521 2011 40 10953 https://doi.org/10.1007/s10953-011-9653-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10953-011-9653-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Xianyong Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut NATIONALLICENCE 700 1- Liu Ronghua Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut NATIONALLICENCE 700 1- Ji Danhong Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut NATIONALLICENCE 700 1- Yang Fengxian Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut NATIONALLICENCE 700 1- Li Xiaofang Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut NATIONALLICENCE 700 1- Xie Jian Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Zhou Jiming Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut NATIONALLICENCE 700 1- Yi Pinggui Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, China aut NATIONALLICENCE 773 0- Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/3(2011-03-01), 521-531 0095-9782 40:3<521 2011 40 10953 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer