caa a22 4500 445328231 CHVBK 20180317142728.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10953-011-9747-6 doi (NATIONALLICENCE)springer-10.1007/s10953-011-9747-6 Probing the Binding of Rifampicin to Bovine Serum Albumin in Aqueous Solution [Elektronische Daten] [Ou-Yang Yu, Yi-Feng Cheng, Shi-Yan Huang, Ai-Min Bai, Yan-Jun Hu] The binding of rifampicin (RFP), an anti-tuberculosis agent, to bovine serum albumin (BSA) was studied at physiological conditions (pH=7.40) by a spectroscopic approach. In the discussion of the quenching mechanism, it was proved that the fluorescence quenching of BSA by RFP is a result of the formation of a RFP-BSA complex. Binding parameters were determined using the modified Stern-Volmer equation and Scatchard's equation to provide a measure of the binding affinity between RFP and BSA. The resulting thermodynamic parameters ΔG, ΔH, and ΔS at different temperatures indicate that electrostatic interactions play a major role in RFP-BSA association. Site marker competitive displacement experiments demonstrate that RFP binds with high affinity to the site I (subdomain IIA) of BSA. Furthermore, the effect of metal ions on the RFP-BSA system was studied, and the specific binding distance r (3.38nm) between donor and acceptor (RFP) was obtained according to the fluorescence resonance energy transfer (FRET). Springer Science+Business Media, LLC, 2011 Rifampicin nationallicence Bovine serum albumin nationallicence Binding parameter nationallicence Site marker nationallicence Metal ion nationallicence Yu Ou-Yang Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut Cheng Yi-Feng Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut Huang Shi-Yan Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut Bai Ai-Min Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut Hu Yan-Jun Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/10(2011-10-01), 1711-1723 0095-9782 40:10<1711 2011 40 10953 https://doi.org/10.1007/s10953-011-9747-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10953-011-9747-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Ou-Yang Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut NATIONALLICENCE 700 1- Cheng Yi-Feng Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut NATIONALLICENCE 700 1- Huang Shi-Yan Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut NATIONALLICENCE 700 1- Bai Ai-Min Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut NATIONALLICENCE 700 1- Hu Yan-Jun Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, 435002, Huangshi, P.R. China aut NATIONALLICENCE 773 0- Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/10(2011-10-01), 1711-1723 0095-9782 40:10<1711 2011 40 10953 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer