caa a22 4500 445328282 CHVBK 20180317142728.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10953-011-9666-6 doi (NATIONALLICENCE)springer-10.1007/s10953-011-9666-6 Interaction Between Plumbagin and Human Serum Albumin by Fluorescence Spectroscopy [Elektronische Daten] [Shuang Li, Di Yao, Hedong Bian, Zhenfeng Chen, Jing Yu, Qing Yu, Hong Liang] The interaction of plumbagin (PLU) with human serum albumin (HSA) in physiological buffer (pH=7.4) was studied by fluorescence spectroscopy. Results obtained from analysis of the fluorescence spectra indicated that PLU has a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. Fluorescence quenching data revealed that the quenching constants (K) are 4.43×104, 3.26×104 and 1.69×104L⋅mol−1 at 293, 303 and 313 K, respectively. The thermodynamic parameters ΔH° and ΔS° were calculated to be −36.63 kJ⋅mol−1, and −35.702 J⋅mol−1⋅K−1 respectively, which suggested that van der Waals interactions and hydrogen bonds play a major role in the interaction of PLU with HSA. The distance between donor (HSA) and acceptor (PLU) was calculated to be 3.76 nm based on Förster's non-radiative energy transfer theory. The results of synchronous fluorescence spectra showed that binding of PLU to HSA can induce conformational changes in HSA. Springer Science+Business Media, LLC, 2011 Human serum albumin nationallicence Plumbagin nationallicence Fluorescence nationallicence Conformational change nationallicence Energy transfer nationallicence Li Shuang The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut Yao Di The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut Bian Hedong The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut Chen Zhenfeng The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut Yu Jing The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut Yu Qing The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut Liang Hong The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/4(2011-04-01), 709-718 0095-9782 40:4<709 2011 40 10953 https://doi.org/10.1007/s10953-011-9666-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10953-011-9666-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Shuang The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut NATIONALLICENCE 700 1- Yao Di The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut NATIONALLICENCE 700 1- Bian Hedong The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut NATIONALLICENCE 700 1- Chen Zhenfeng The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut NATIONALLICENCE 700 1- Yu Jing The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut NATIONALLICENCE 700 1- Yu Qing The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut NATIONALLICENCE 700 1- Liang Hong The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, 541004, Guilin, P.R. China aut NATIONALLICENCE 773 0- Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/4(2011-04-01), 709-718 0095-9782 40:4<709 2011 40 10953 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer