caa a22 4500 445328320 CHVBK 20180317142729.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10953-011-9668-4 doi (NATIONALLICENCE)springer-10.1007/s10953-011-9668-4 Thermodynamic Study of the Binding of Mercury Ion to Human Growth Hormone at Different Temperatures [Elektronische Daten] [E. Lemeski, G. Behbehani, A. Saboury, M. Monajjemi, R. Mehrabian, M. Golsefidi, H. Rajabzadeh, M. Baei, S. Hasanzadeh] The interaction of human growth hormone (hGH) with the divalent mercury ion was studied by isothermal titration calorimetry at two temperatures of 27 °C and 37 °C in aqueous solutions. We found that there is a set of two identical and non-interacting binding sites for Hg2+ ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 4.2 mmol⋅L−1 and −14.8 kJ⋅mol−1 at 27 °C and 5.1 mmol⋅L−1 and −14.2kJ⋅mol−1 at 37 °C, respectively. The results obtained indicate that the stability of the protein increases due to the binding of mercury ions using the extended solvation theory. Springer Science+Business Media, LLC, 2011 Human growth hormone (hGH) nationallicence Isothermal titration calorimetry (ITC) nationallicence Solvation model nationallicence Mercury ion nationallicence Metal binding nationallicence Lemeski E. Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran aut Behbehani G. Department of Chemistry, Takestan Branch, Islamic Azad University, Takestan, Iran aut Saboury A. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran aut Monajjemi M. Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran aut Mehrabian R. Department of Chemistry, Gorgan Branch, Islamic Azad University, Gorgan, Iran aut Golsefidi M. Department of Chemistry, Gorgan Branch, Islamic Azad University, Gorgan, Iran aut Rajabzadeh H. Department of Chemistry, Dezful Branch, Islamic Azad University, Dezful, Iran aut Baei M. Minoodasht Branch, Islamic Azad University, Minoodasht, Iran aut Hasanzadeh S. Young Researchers Club, Gorgan Branch, Islamic Azad University, Gorgan, Iran aut Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/4(2011-04-01), 575-586 0095-9782 40:4<575 2011 40 10953 https://doi.org/10.1007/s10953-011-9668-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10953-011-9668-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lemeski E. Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran aut NATIONALLICENCE 700 1- Behbehani G. Department of Chemistry, Takestan Branch, Islamic Azad University, Takestan, Iran aut NATIONALLICENCE 700 1- Saboury A. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran aut NATIONALLICENCE 700 1- Monajjemi M. Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran aut NATIONALLICENCE 700 1- Mehrabian R. Department of Chemistry, Gorgan Branch, Islamic Azad University, Gorgan, Iran aut NATIONALLICENCE 700 1- Golsefidi M. Department of Chemistry, Gorgan Branch, Islamic Azad University, Gorgan, Iran aut NATIONALLICENCE 700 1- Rajabzadeh H. Department of Chemistry, Dezful Branch, Islamic Azad University, Dezful, Iran aut NATIONALLICENCE 700 1- Baei M. Minoodasht Branch, Islamic Azad University, Minoodasht, Iran aut NATIONALLICENCE 700 1- Hasanzadeh S. Young Researchers Club, Gorgan Branch, Islamic Azad University, Gorgan, Iran aut NATIONALLICENCE 773 0- Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/4(2011-04-01), 575-586 0095-9782 40:4<575 2011 40 10953 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer