caa a22 4500 445328630 CHVBK 20180317142730.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10953-011-9686-2 doi (NATIONALLICENCE)springer-10.1007/s10953-011-9686-2 A Thermodynamic Study of Zinc Ion Interaction with Bovine Carbonic Anhydrase II at Different Temperatures [Elektronische Daten] [L. Barzegar, G. RezaeiBehbehani, A. Saboury] A thermodynamic study of the interaction of bovine carbonic anhydrase II, CAII, with zinc ion was carried out by using isothermal titration calorimetry (ITC) at 300, 310 and 320K in Tris buffer solutions at pH=7.5. The heats of Zn2++ CAII interaction are reported and analyzed in terms of the new solvation theory. It was indicated that there are three identical and non-cooperative sites on CAII for Zn2+. The binding of a zinc ion is exothermic with dissociation equilibrium constants of 78.32, 95.81 and 116.70mmol⋅L−1 at 300, 310 and 320K respectively. Springer Science+Business Media, LLC, 2011 Bovine carbonic anhydrase nationallicence Zinc ion nationallicence Isothermal titration calorimetry nationallicence Barzegar L. Chemistry Department, Faculty of Science, Islamic Azad University, Takestan Branch, Takestan, Iran aut RezaeiBehbehani G. Chemistry Department, Faculty of Science, Islamic Azad University, Takestan Branch, Takestan, Iran aut Saboury A. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran aut Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/5(2011-05-01), 843-848 0095-9782 40:5<843 2011 40 10953 https://doi.org/10.1007/s10953-011-9686-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10953-011-9686-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barzegar L. Chemistry Department, Faculty of Science, Islamic Azad University, Takestan Branch, Takestan, Iran aut NATIONALLICENCE 700 1- RezaeiBehbehani G. Chemistry Department, Faculty of Science, Islamic Azad University, Takestan Branch, Takestan, Iran aut NATIONALLICENCE 700 1- Saboury A. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran aut NATIONALLICENCE 773 0- Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/5(2011-05-01), 843-848 0095-9782 40:5<843 2011 40 10953 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer