caa a22 4500 445328665 CHVBK 20180317142730.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10953-010-9647-1 doi (NATIONALLICENCE)springer-10.1007/s10953-010-9647-1 Characterization of Phenosafranine-Hemoglobin Interactions in Aqueous Solution [Elektronische Daten] [Wei Liu, Fei Ding, Ying Sun] Binding of the drug phenosafranine to hemoglobin (Hb) in aqueous solutions was investigated by fluorescence, UV/vis and circular dichroism (CD) spectral methods at pH=7.4. The fluorescence data showed that fluorescence quenching of Hb by phenosafranine is the result of formation of a phenosafranine-Hb complex with a 1:1 molar ratio. Thermodynamic analysis implied that hydrophobic, electrostatic and hydrogen bond interactions are all involved in stabilizing the complex. The molecular distance (r=4.29 nm) between the donor (Hb) and acceptor (phenosafranine) was calculated according to Förster's theory. The features of phenosafranine-induced secondary structure changes of Hb have been studied by synchronous fluorescence, CD and three-dimensional fluorescence spectroscopy. This study improves our knowledge of the interaction dynamics of phenazinium drugs to the physiologically important protein Hb. Springer Science+Business Media, LLC, 2011 Phenosafranine nationallicence Hemoglobin nationallicence Fluorescence nationallicence Circular dichroism nationallicence Ligand binding nationallicence Hb : hemoglobin nationallicence Trp : tryptophan nationallicence Tyr : tyrosine nationallicence Phe : phenylalanine nationallicence CD : circular dichroism nationallicence Liu Wei College of Economics and Management, China Agricultural University, 100083, Beijing, China aut Ding Fei Department of Chemistry, China Agricultural University, 100193, Beijing, China aut Sun Ying Department of Chemistry, China Agricultural University, 100193, Beijing, China aut Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/2(2011-02-01), 231-246 0095-9782 40:2<231 2011 40 10953 https://doi.org/10.1007/s10953-010-9647-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10953-010-9647-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wei College of Economics and Management, China Agricultural University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Ding Fei Department of Chemistry, China Agricultural University, 100193, Beijing, China aut NATIONALLICENCE 700 1- Sun Ying Department of Chemistry, China Agricultural University, 100193, Beijing, China aut NATIONALLICENCE 773 0- Journal of Solution Chemistry Springer US; http://www.springer-ny.com 40/2(2011-02-01), 231-246 0095-9782 40:2<231 2011 40 10953 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer