caa a22 4500 445330619 CHVBK 20180317142736.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10689-010-9396-8 doi (NATIONALLICENCE)springer-10.1007/s10689-010-9396-8 Gene expression in response to ionizing radiation and family history of gastric cancer [Elektronische Daten] [Francesca Marcon, Francesco Silvestrini, Ester Siniscalchi, Domenico Palli, Calogero Saieva, Riccardo Crebelli] Genes and molecular pathways involved in familial clustering of gastric cancer have not yet been identified. The purpose of the present study was to investigate gene expression changes in response to a cellular stress, and its link with a positive family history for this neoplasia. To this aim leukocytes of healthy first-degree relatives of gastric cancer patients and controls were challenged in vitro with ionizing radiation and gene expression evaluated 4h later on microarrays with 1,800 cancer-related genes. Eight genes, mainly involved in signal transduction and cell cycle regulation, were differentially expressed in healthy relatives of gastric cancer cases. Functional class scoring by Gene Ontology classification highlighted two G-protein related pathways, implicated in the proliferation of neoplastic tissue, which were differentially expressed in healthy subjects with positive family history of gastric cancer. The relative expression of 84 genes related to these pathways was examined using the SYBR green-based quantitative real-time PCR. The results confirmed the indication of an involvement of G-protein coupled receptor pathways in GC familiarity provided by microarray analysis. This study indicates a possible association between familiarity for gastric cancer and altered transcriptional response to ionizing radiation. Springer Science+Business Media B.V., 2010 Gastric cancer nationallicence Ionising radiation nationallicence Gene expression nationallicence Hereditary nationallicence Blood cells nationallicence Familial nationallicence Marcon Francesca Department of Environment and Primary Prevention, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy aut Silvestrini Francesco Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy aut Siniscalchi Ester Department of Environment and Primary Prevention, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy aut Palli Domenico Molecular and Nutritional Epidemiology Unit, ISPO-Cancer Prevention and Research Institute, Via Cosimo il Vecchio 2, 50139, Florence, Italy aut Saieva Calogero Molecular and Nutritional Epidemiology Unit, ISPO-Cancer Prevention and Research Institute, Via Cosimo il Vecchio 2, 50139, Florence, Italy aut Crebelli Riccardo Department of Environment and Primary Prevention, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy aut Familial Cancer Springer Netherlands 10/1(2011-03-01), 107-118 1389-9600 10:1<107 2011 10 10689 https://doi.org/10.1007/s10689-010-9396-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10689-010-9396-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marcon Francesca Department of Environment and Primary Prevention, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Silvestrini Francesco Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Siniscalchi Ester Department of Environment and Primary Prevention, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Palli Domenico Molecular and Nutritional Epidemiology Unit, ISPO-Cancer Prevention and Research Institute, Via Cosimo il Vecchio 2, 50139, Florence, Italy aut NATIONALLICENCE 700 1- Saieva Calogero Molecular and Nutritional Epidemiology Unit, ISPO-Cancer Prevention and Research Institute, Via Cosimo il Vecchio 2, 50139, Florence, Italy aut NATIONALLICENCE 700 1- Crebelli Riccardo Department of Environment and Primary Prevention, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy aut NATIONALLICENCE 773 0- Familial Cancer Springer Netherlands 10/1(2011-03-01), 107-118 1389-9600 10:1<107 2011 10 10689 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer