caa a22 4500 44533262X CHVBK 20180317142742.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1245/s10434-010-1213-y doi (NATIONALLICENCE)springer-10.1245/s10434-010-1213-y MiRNAs and Their Association with Locoregional Staging and Survival Following Surgery for Esophageal Carcinoma [Elektronische Daten] [Richard Hummel, Damian Hussey, Michael Michael, Joerg Haier, Matthias Bruewer, Norbert Senninger, David Watson] Background: Prognostic and staging information for esophageal cancer impacts clinical decision making. miRNAs, a newly discovered class of biomarkers and their expression might add additional information relevant to this. In this study we evaluated the expression of selected miRNAs and their relationship to tumor stage and survival in patients with locally advanced tumors following esophagectomy. Materials and Methods: A total of 43 individuals undergoing esophagectomy (without neoadjuvant therapy) for locally advanced but not metastatic (pT2/3; pN0/1) disease (22 adenocarcinoma [EAC], 21 squamous cell carcinoma [SCC]) were included in this study. Perioperative clinical and survival data were collected and managed on a database. The expression of miR-21, miR-106a, miR-148a, miR-205 in formalin-fixed paraffin-embedded specimens was evaluated by TaqMan qPCR assays. Expression was compared with clinicopathological features of the cancers and outcome. Results: In EAC, miR-148a expression levels were inversely associated with cancer differentiation. miR-21 expression levels were higher in SCC if distant lymph node metastases were present. miR-148a levels were lower when EAC was more proximally located, and miR-21 levels were lower when SCC was more proximal. miR-106a and miR-148a were lower in patients with SCC who developed recurrent disease or had a tumor-related death. Conclusions: In patients with locally advanced esophageal squamous cell carcinoma, but not adenocarcinoma, alterations in the expression of miR-21 correlate with tumor location and lymph node status. Furthermore, miR-106a and miR-148a expression correlates with disease recurrence and tumor-related mortality. miRNA markers might inform the initial assessment of these patients, and predict those at higher risk of postsurgical recurrence. Society of Surgical Oncology, 2010 Hummel Richard Department of Surgery, Flinders University, Bedford Park, SA, Australia aut Hussey Damian Department of Surgery, Flinders University, Bedford Park, SA, Australia aut Michael Michael Department of Gastroenterology and Hepatology, Flinders University, Bedford Park, SA, Australia aut Haier Joerg Comprehensive Cancer Centre, University of Muenster, Muenster, Germany aut Bruewer Matthias Department of General and Visceral Surgery, University of Muenster, Muenster, Germany aut Senninger Norbert Department of General and Visceral Surgery, University of Muenster, Muenster, Germany aut Watson David Department of Surgery, Flinders University, Bedford Park, SA, Australia aut Annals of Surgical Oncology Springer-Verlag 18/1(2011-01-01), 253-260 1068-9265 18:1<253 2011 18 10434 https://doi.org/10.1245/s10434-010-1213-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1213-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hummel Richard Department of Surgery, Flinders University, Bedford Park, SA, Australia aut NATIONALLICENCE 700 1- Hussey Damian Department of Surgery, Flinders University, Bedford Park, SA, Australia aut NATIONALLICENCE 700 1- Michael Michael Department of Gastroenterology and Hepatology, Flinders University, Bedford Park, SA, Australia aut NATIONALLICENCE 700 1- Haier Joerg Comprehensive Cancer Centre, University of Muenster, Muenster, Germany aut NATIONALLICENCE 700 1- Bruewer Matthias Department of General and Visceral Surgery, University of Muenster, Muenster, Germany aut NATIONALLICENCE 700 1- Senninger Norbert Department of General and Visceral Surgery, University of Muenster, Muenster, Germany aut NATIONALLICENCE 700 1- Watson David Department of Surgery, Flinders University, Bedford Park, SA, Australia aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/1(2011-01-01), 253-260 1068-9265 18:1<253 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer