caa a22 4500 445332964 CHVBK 20180317142743.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1245/s10434-010-1289-4 doi (NATIONALLICENCE)springer-10.1245/s10434-010-1289-4 Low Expression of Claudin-4 is Associated with Poor Prognosis in Esophageal Squamous Cell Carcinoma [Elektronische Daten] [Chang Sung, Song Han, Seok-Hyung Kim] Background: Claudins are 22-27kDa sized adhesion molecules that constitute tight junctions. Their expression levels are often tissue-specific, and their altered degrees of expression have been reported in a variety of cancers. In addition, the prognostic significance of claudin expression has been implicated in various human cancers. However, the prognostic significance of claudin-4 expression in esophageal squamous cell carcinoma (ESCC) remains to be clarified. Materials and Methods: We investigated the prognostic significance of claudin-4 expression in 164 cases of ESCC using immunohistochemisty. We also evaluated claudin-4 mRNA expression levels using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) and analyzed its specific promoter methylation status using quantitative methylation-specific PCR. Results: According to clinicopathological parameters, low claudin-4 expression was found to be significantly associated with histological differentiation (P=0.003), invasion depth (P=0.002), and lymph node metastasis (P=0.024). Low claudin-4 expression showed unfavorable influences on disease-free survival (P=0.0115) and overall survival (OS) (P=0.0009). In multivariate analysis, low claudin-4 expression was an independent predictor of poor OS (P=0.007). Claudin-4 mRNA levels assessed using real-time RT-PCR were consistent with the protein levels determined using immunohistochemistry. Furthermore, this study demonstrates that loss of claudin-4 is associated with promoter hypermethylation. Conclusions: Our study indicates that claudin-4 expression is deregulated in ESCC, implying its potential use as a prognostic biomarker in ESCC. Society of Surgical Oncology, 2010 Sung Chang Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Han Song Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Kim Seok-Hyung Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Annals of Surgical Oncology Springer-Verlag 18/1(2011-01-01), 273-281 1068-9265 18:1<273 2011 18 10434 https://doi.org/10.1245/s10434-010-1289-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1289-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sung Chang Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Han Song Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Seok-Hyung Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/1(2011-01-01), 273-281 1068-9265 18:1<273 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer