caa a22 4500 445333049 CHVBK 20180317142743.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1245/s10434-010-1271-1 doi (NATIONALLICENCE)springer-10.1245/s10434-010-1271-1 STC2: A Predictive Marker for Lymph Node Metastasis in Esophageal Squamous-Cell Carcinoma [Elektronische Daten] [Yoshiaki Kita, Koshi Mimori, Masaaki Iwatsuki, Takehiko Yokobori, Keisuke Ieta, Fumiaki Tanaka, Hideshi Ishii, Hiroshi Okumura, Shoji Natsugoe, Masaki Mori] Background: We sought to identify genes associated with the progression and metastasis of esophageal squamous-cell cancer by comparing the expression profiles of normal, primary cancer, and metastatic cancer cells isolated with laser microdissection. Methods: Oligo microarray analysis identified several lymph node-specific, metastasis-related genes. STC2 (stanniocalcin 2), which was overexpressed in esophageal cancer cases, was chosen for further characterization. Quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry were used to explore the clinicopathologic significance of STC2 expression status in 70 cases. Additionally, the functional role of STC2 in esophageal cancer was studied by the attenuation of STC2 in an esophageal cancer cell line. Results: Laser microdissection and oligo microarray analysis identified 63 candidate genes. Among them, STC2 showed higher expression in cancer tissue than in corresponding normal tissue (P < 0.001). STC2 expression was significantly correlated with lymph node metastasis, lymphatic invasion, and distant metastasis (P=0.005, 0.007, and 0.038, respectively). Patients whose tumors had high STC2 expression had a worse 5-year survival rate than patients whose tumors had a low STC2 expression level (P=0.016). STC2 transfected cells had a significantly higher proliferation rate than control cells (P < 0.001). Additionally, STC2 transfected cells were more invasive in vitro (P < 0.001) than control cells. These findings were validated by means of RNA interference assays. Conclusions: We identified lymph node-specific, metastasis-related genes in esophageal cancer cells. One of these, STC2, may be associated with lymph node metastasis, making it a potential prognostic marker for esophageal cancer patients. Society of Surgical Oncology, 2010 Kita Yoshiaki Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut Mimori Koshi Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut Iwatsuki Masaaki Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut Yokobori Takehiko Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut Ieta Keisuke Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut Tanaka Fumiaki Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut Ishii Hideshi Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut Okumura Hiroshi Department of Surgical Oncology and Digestive Surgery, Field of Oncology, Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Science, Kagoshima, Japan aut Natsugoe Shoji Department of Surgical Oncology and Digestive Surgery, Field of Oncology, Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Science, Kagoshima, Japan aut Mori Masaki Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan aut Annals of Surgical Oncology Springer-Verlag 18/1(2011-01-01), 261-272 1068-9265 18:1<261 2011 18 10434 https://doi.org/10.1245/s10434-010-1271-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1271-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kita Yoshiaki Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut NATIONALLICENCE 700 1- Mimori Koshi Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut NATIONALLICENCE 700 1- Iwatsuki Masaaki Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut NATIONALLICENCE 700 1- Yokobori Takehiko Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut NATIONALLICENCE 700 1- Ieta Keisuke Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut NATIONALLICENCE 700 1- Tanaka Fumiaki Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut NATIONALLICENCE 700 1- Ishii Hideshi Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan aut NATIONALLICENCE 700 1- Okumura Hiroshi Department of Surgical Oncology and Digestive Surgery, Field of Oncology, Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Science, Kagoshima, Japan aut NATIONALLICENCE 700 1- Natsugoe Shoji Department of Surgical Oncology and Digestive Surgery, Field of Oncology, Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Science, Kagoshima, Japan aut NATIONALLICENCE 700 1- Mori Masaki Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/1(2011-01-01), 261-272 1068-9265 18:1<261 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer