caa a22 4500 445333081 CHVBK 20180317142743.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1245/s10434-010-1225-7 doi (NATIONALLICENCE)springer-10.1245/s10434-010-1225-7 Pancreatic Cyst Fluid and Serum Mucin Levels Predict Dysplasia in Intraductal Papillary Mucinous Neoplasms of the Pancreas [Elektronische Daten] [Ajay Maker, Nora Katabi, Mithat Gonen, Ronald DeMatteo, Michael D'Angelica, Yuman Fong, William Jarnagin, Murray Brennan, Peter Allen] Introduction: There are no reliable markers of dysplasia in patients with incidentally discovered intraductal papillary mucinous neoplasms of the pancreas (IPMN). IPMN dysplasia may be associated with mucin protein (MUC) expression and histopathologic subtype. We hypothesize that MUC expression in cyst fluid and serum can identify lesions with high risk of malignancy. Methods: Cyst fluid and serum were collected from 40 patients during pancreatectomy for IPMN between 2005 and 2009. Samples were grouped into low-risk (low-grade or moderate dysplasia, n=21) and high-risk groups (high-grade dysplasia or carcinoma, n=19). Mucin expression (MUC1, MUC2, MUC4, and MUC5AC) was assessed utilizing enzyme-linked immunosorbent assays. Results: MUC2 and MUC4 cyst fluid concentrations were elevated in high-risk versus low-risk groups (10±3.0ng/ml vs. 4.4±1.2ng/ml, p=0.03; 20.6±10.6ng/ml vs. 4.5±1.4ng/ml, p=0.03, respectively). Corresponding serum samples revealed higher levels of MUC5AC in high-risk compared with low-risk patients (19.9±9.3ng/ml vs. 2.2±1.1ng/ml, p=0.04). Histopathologic subtype was significantly associated with grade of dysplasia, and the intestinal subtype displayed increased MUC2 cyst fluid concentrations (13.8±6.5ng/ml vs. 4.1±0.9ng/ml, p=0.02). Conclusions: In this study, high-risk IPMN showed elevated cyst fluid concentrations of MUC2 and MUC4, and increased serum levels of MUC5AC. High-risk IPMN also displayed a distinct mucin expression profile in specific histologic subtypes. These data, if validated, may allow surgeons to more appropriately select patients for operative resection. Society of Surgical Oncology, 2010 Maker Ajay Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut Katabi Nora Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut Gonen Mithat Department of Epidemiology and Biostatistics, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut DeMatteo Ronald Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut D'Angelica Michael Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut Fong Yuman Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut Jarnagin William Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut Brennan Murray Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut Allen Peter Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut Annals of Surgical Oncology Springer-Verlag 18/1(2011-01-01), 199-206 1068-9265 18:1<199 2011 18 10434 https://doi.org/10.1245/s10434-010-1225-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1225-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maker Ajay Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 700 1- Katabi Nora Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 700 1- Gonen Mithat Department of Epidemiology and Biostatistics, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 700 1- DeMatteo Ronald Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 700 1- D'Angelica Michael Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 700 1- Fong Yuman Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 700 1- Jarnagin William Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 700 1- Brennan Murray Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 700 1- Allen Peter Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/1(2011-01-01), 199-206 1068-9265 18:1<199 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer