caa a22 4500 445333375 CHVBK 20180317142744.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1245/s10434-011-1558-x doi (NATIONALLICENCE)springer-10.1245/s10434-011-1558-x Senescence-Associated Gene YPEL3 Is Downregulated in Human Colon Tumors [Elektronische Daten] [Rebecca Tuttle, Margo Simon, David Hitch, J. Maiorano, Minia Hellan, James Ouellette, Paula Termuhlen, Steven Berberich] Background: Previous work has demonstrated YPEL3 to be a growth-suppressive protein that acts through a pathway of cellular senescence. We set out to determine whether human colon tumors demonstrated downregulation of YPEL3. Methods: We collected colon tumor samples with matched normal control samples and analyzed them for YPEL3 gene expression by reverse transcriptase-polymerase chain reaction and CpG hypermethylation of the YPEL3 promoter by base-specific polymerase chain reaction analysis. Colon cancer cell lines (Caco-2 and HCT116−/− p53) were used to assess YPEL3 gene expression after treatment with 5-azadeoxycytidine or trichostatin A. Results: Reverse transcriptase-polymerase chain reaction analysis demonstrated a decrease in YPEL3 expression in tumor samples when compared to their patient-matched normal tissue. We determined that DNA methylation of the YPEL3 promoter CpG island does not play a role in YPEL3 regulation in human colon tumors or colon cancer cells lines, consistent with the inability of 5-azadeoxycytidine treatment to induce YPEL3 expression in colon cancer cell lines. In contrast, colon cell line results suggest that histone acetylation may play a role in YPEL3 regulation in colon cancer. Conclusions: YPEL3 is preferentially downregulated in human colon adenocarcinomas. DNA hypermethylation does not appear to be the mechanism of YPEL3 downregulation in this subset of collected patient samples or in colon cell lines. Histone acetylation may be a relevant epigenetic modulator of YPEL3 in colon adenocarcinomas. Future investigation of YPEL3 and its role in colon cancer signaling and development may lead to increased understanding and alternative treatment options for this disease. Society of Surgical Oncology, 2011 Tuttle Rebecca Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut Simon Margo Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut Hitch David Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut Maiorano J. Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut Hellan Minia Department of Surgery, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut Ouellette James Department of Surgery, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut Termuhlen Paula Department of Surgery, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut Berberich Steven Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut Annals of Surgical Oncology Springer-Verlag 18/6(2011-06-01), 1791-1796 1068-9265 18:6<1791 2011 18 10434 https://doi.org/10.1245/s10434-011-1558-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1558-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tuttle Rebecca Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut NATIONALLICENCE 700 1- Simon Margo Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut NATIONALLICENCE 700 1- Hitch David Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut NATIONALLICENCE 700 1- Maiorano J. Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut NATIONALLICENCE 700 1- Hellan Minia Department of Surgery, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut NATIONALLICENCE 700 1- Ouellette James Department of Surgery, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut NATIONALLICENCE 700 1- Termuhlen Paula Department of Surgery, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut NATIONALLICENCE 700 1- Berberich Steven Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/6(2011-06-01), 1791-1796 1068-9265 18:6<1791 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer