caa a22 4500 445333464 CHVBK 20180317142745.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1245/s10434-010-1504-3 doi (NATIONALLICENCE)springer-10.1245/s10434-010-1504-3 Nicotine Enhances Colon Cancer Cell Migration by Induction of Fibronectin [Elektronische Daten] [Po-Li Wei, Li-Jen Kuo, Ming-Te Huang, Wen-Chien Ting, Yuan-Soon Ho, Weu Wang, Jane An, Yu-Jia Chang] Background: Long-term cigarette smoking increases the risk of colorectal cancer mortality. Tobacco's addictive toxin, nicotine, was reported to increase DNA synthesis of colon cancer cells. Because metastasis is the major cause of cancer death, the influence of nicotine on the migration of colon cancer cells remains to be determined. Methods: The influence of nicotine on the migration of colon cancer cells was evaluated using transwell assay. Nicotine receptor-mediated migration was studied by using both inhibitors and small interfering RNA (siRNA). The role of COX-2 signal was studied using pharmacological inhibitors. The expression of epithelial mesenchymal transition (EMT) marker and COX-2 signal was evaluated using real-time polymerase chain reaction (PCR). Results: Nicotine enhanced DLD-1 and SW480 cell migration in a dose-dependent manner. We used inhibitors and siRNA to demonstrate that α7-nAChR mediates nicotine-enhanced colon cancer cell migration and upregulates fibronectin expression, which is involved in nicotine-enhanced migration. Furthermore, COX-2 signal was induced by nicotine treatment and is involved in nicotine-enhanced fibronectin expression. Conclusions: Nicotine, tobacco's additive toxin, enhances colon cancer metastasis through α7-nAChR and fibronectin—a mesenchymal marker for epithelial mesenchymal transition. Furthermore, COX-2 signal was involved in the induction of fibronectin. Therefore, smoking may play role in the progression of colon cancer. Society of Surgical Oncology, 2011 Wei Po-Li Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Kuo Li-Jen Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Huang Ming-Te Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan aut Ting Wen-Chien Division of Colorectal Surgery, Department of Surgery, Chung Shan Medical Hospital, Taichung, Taiwan aut Ho Yuan-Soon School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan aut Wang Weu Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut An Jane Westchester Medical Center, Hawthorne, NY, USA aut Chang Yu-Jia Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Annals of Surgical Oncology Springer-Verlag 18/6(2011-06-01), 1782-1790 1068-9265 18:6<1782 2011 18 10434 https://doi.org/10.1245/s10434-010-1504-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1504-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Po-Li Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Kuo Li-Jen Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Huang Ming-Te Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Ting Wen-Chien Division of Colorectal Surgery, Department of Surgery, Chung Shan Medical Hospital, Taichung, Taiwan aut NATIONALLICENCE 700 1- Ho Yuan-Soon School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Wang Weu Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- An Jane Westchester Medical Center, Hawthorne, NY, USA aut NATIONALLICENCE 700 1- Chang Yu-Jia Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/6(2011-06-01), 1782-1790 1068-9265 18:6<1782 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer