caa a22 4500 445333480 CHVBK 20180317142745.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1245/s10434-010-1500-7 doi (NATIONALLICENCE)springer-10.1245/s10434-010-1500-7 Tissue hOGG1 Genotype Predicts Bladder Cancer Prognosis: A Novel Approach Using a Peptide Nucleic Acid Clamping Method [Elektronische Daten] [Yun-Sok Ha, Chunri Yan, Isaac Kim, Seok-Joong Yun, Sung-Kwon Moon, Wun-Jae Kim] Background: Tissue genotyping is a more useful approach than using blood genomic DNA, because the tumor tissues can reflect the effects of somatic mutations in cancer. We investigated the value of the human oxoguanine glycosylase (hOGG1) genotype determined in tumor tissues as a prognostic indicator for bladder cancer (BC) using a novel technological approach. Methods: A total of 335 DNA samples from patients with primary BC were analyzed by peptide nucleic acid (PNA)-mediated real-time polymerase chain reaction (PCR) clamping to characterize the association between genetic polymorphisms within hOGG1 codon 326 and the clinicopathological characteristics of primary BC patients. Results: Tumor stage and number were significantly associated with the hOGG1 codon 326 genotype in nonmuscle invasive bladder cancer (NMIBC) patients. Compared with Cys326Ser and Ser326Ser, the Cys326Cys genotype had a greater progression-free survival benefit in patients with muscle invasive bladder cancer (MIBC). Univariate and multivariate Cox regression analyses indicated that the hOGG1 Cys326Cys genotype has a protective effect against progression in MIBC (hazard ratio, 0.360 and 0.314, respectively). Conclusions: The hOGG1 tissue genotype is associated with aggressive clinicopathological features in NMIBC and with progression in patients with MIBC. Results suggest that the hOGG1 tissue genotype represents a promising marker for assessing BC prognosis in the clinical setting. Society of Surgical Oncology, 2010 Ha Yun-Sok Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea aut Yan Chunri Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea aut Kim Isaac Section of Urologic Oncology, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, New Brunswick, NJ, USA aut Yun Seok-Joong Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea aut Moon Sung-Kwon Department of Food and Biotechnology, Chungju National University, Chungju, Chungbuk, South Korea aut Kim Wun-Jae Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea aut Annals of Surgical Oncology Springer-Verlag 18/6(2011-06-01), 1775-1781 1068-9265 18:6<1775 2011 18 10434 https://doi.org/10.1245/s10434-010-1500-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1500-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ha Yun-Sok Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea aut NATIONALLICENCE 700 1- Yan Chunri Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea aut NATIONALLICENCE 700 1- Kim Isaac Section of Urologic Oncology, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Yun Seok-Joong Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea aut NATIONALLICENCE 700 1- Moon Sung-Kwon Department of Food and Biotechnology, Chungju National University, Chungju, Chungbuk, South Korea aut NATIONALLICENCE 700 1- Kim Wun-Jae Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/6(2011-06-01), 1775-1781 1068-9265 18:6<1775 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer