caa a22 4500 445333820 CHVBK 20180317142746.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1245/s10434-011-1598-2 doi (NATIONALLICENCE)springer-10.1245/s10434-011-1598-2 Nicotine Promotes Cell Migration Through Alpha7 Nicotinic Acetylcholine Receptor in Gastric Cancer Cells [Elektronische Daten] [Yung-Chang Lien, Weu Wang, Li-Jen Kuo, Jun-Jen Liu, Po-Li Wei, Yuan-Soon Ho, Wen-Chien Ting, Chih-Hsiung Wu, Yu-Jia Chang] Background: The objective was to study the mechanism of nicotine-enhanced migration of gastric cancer cells. Long-term cigarette smoking increases the risk of gastric cancer mortality. Tobacco-specific mitogen, nicotine, was reported to correlate with cancer progression on gastric cancer. Since metastasis is the major cause of cancer death, the influence of nicotine on the migration of gastric cancer cells remains to be determined. Materials and Methods: The influence of nicotine on migration of gastric cancer cells was evaluated by transwell assay and wound-healing migration assay. Receptor-mediated migration was studied by both inhibitor and small interfering RNA. Results: Alpha7 nicotinic acetylcholine receptor, alpha7-nAChR, was identified in gastric cancer cell lines, AGS cells. Nicotine enhanced AGS cell migration in transwell assay and wound-healing migration assay in a dose-dependent manner. We used inhibitor and siRNA to demonstrate that alpha7-nAChR mediated nicotine-enhanced gastric cancer cell migration through downregulation E-cadherin and upregulation ZEB-1 and snail. Conclusions: Tobacco-specific mitogen, nicotine, enhanced gastric cancer metastasis through alpha7-nAChR and suppression of E-cadherin level—one of the hallmarks of epithelial to mesenchymal transition. Therefore, patients with gastric cancer should avoid smoking. Society of Surgical Oncology, 2011 Lien Yung-Chang Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Wang Weu Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Kuo Li-Jen Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Liu Jun-Jen School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan aut Wei Po-Li Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Ho Yuan-Soon School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan aut Ting Wen-Chien Division of Colorectal Surgery, Department of Surgery, Chung Shan Medical University Hospital, Taichung City, Taiwan aut Wu Chih-Hsiung Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Chang Yu-Jia Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut Annals of Surgical Oncology Springer-Verlag 18/9(2011-09-01), 2671-2679 1068-9265 18:9<2671 2011 18 10434 https://doi.org/10.1245/s10434-011-1598-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1598-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lien Yung-Chang Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Wang Weu Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Kuo Li-Jen Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Liu Jun-Jen School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Wei Po-Li Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Ho Yuan-Soon School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Ting Wen-Chien Division of Colorectal Surgery, Department of Surgery, Chung Shan Medical University Hospital, Taichung City, Taiwan aut NATIONALLICENCE 700 1- Wu Chih-Hsiung Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Chang Yu-Jia Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/9(2011-09-01), 2671-2679 1068-9265 18:9<2671 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer