caa a22 4500 445334118 CHVBK 20180317142747.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1245/s10434-011-1567-9 doi (NATIONALLICENCE)springer-10.1245/s10434-011-1567-9 Msi-1 is a Predictor of Survival and a Novel Therapeutic Target in Colon Cancer [Elektronische Daten] [Dawei Li, Xiao Peng, Dongwang Yan, Huamei Tang, Fei Huang, Yinxue Yang, Zhihai Peng] Background: Musashi1 (Msi-1), a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, aberrant expression of Msi-1 has been detected in several malignancies. However, its role in the progression of colon cancer is largely unknown. Materials and Methods: We used Western blotting to examine Msi-1 protein expression in 8 cases of primary colon cancer lesions and paired normal colonic mucosa. Msi-1 expression and clinicopathological significance were determined by immunohistochemical staining in a tissue microarray (TMA) containing 203 cases of primary colon cancer paired with noncancerous tissue and 66 lymph node metastasis (LNM) tissues. RNAi was used to analyze the biological function of Msi-1 in vitro. Results: LNM tissue exhibited a striking increase in Msi-1 expression when compared with primary colon cancer and adjacent normal mucosa (87.9% vs. 64.5% vs. 16.7%, P<.001). Overexpression of Msi-1 was associated with higher clinical stage, T stage, lymph node metastasis, presence of distant metastasis, and Ki-67 positivity. Msi-1 served as an independent prognostic marker whose expression levels correlated with poorer metastasis-free survival (MFS) (HR 5.4; P<.001) and poorer overall survival (OS) (HR 3.8; P<.001). Msi-1 silencing significantly inhibited proliferation ability and attenuated the migration and invasion activity of colon cancer cells. Conclusions: Our study provides the basis to explore the use of Msi-1 as a novel prognostic biomarker in colon cancer patients. Aberrant overexpression of Msi-1 during metastasis of colon cancer also suggests that it is a potential therapeutic target. Society of Surgical Oncology, 2011 Li Dawei Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut Peng Xiao Department of Clinical Medicine, Xiangya School of Medicine, Central South University, Changsha, People's Republic of China aut Yan Dongwang Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut Tang Huamei Department of Pathology, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut Huang Fei Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut Yang Yinxue Department of Colorectal Surgery, Affiliated Hospital of Ningxia Medical University, Ningxia, People's Republic of China aut Peng Zhihai Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut Annals of Surgical Oncology Springer-Verlag 18/7(2011-07-01), 2074-2083 1068-9265 18:7<2074 2011 18 10434 https://doi.org/10.1245/s10434-011-1567-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1567-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Dawei Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Peng Xiao Department of Clinical Medicine, Xiangya School of Medicine, Central South University, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Yan Dongwang Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Tang Huamei Department of Pathology, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Huang Fei Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yang Yinxue Department of Colorectal Surgery, Affiliated Hospital of Ningxia Medical University, Ningxia, People's Republic of China aut NATIONALLICENCE 700 1- Peng Zhihai Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/7(2011-07-01), 2074-2083 1068-9265 18:7<2074 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer