caa a22 4500 445334606 CHVBK 20180317142750.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1245/s10434-011-1730-3 doi (NATIONALLICENCE)springer-10.1245/s10434-011-1730-3 Carbonic Anhydrase IX Overexpression is Associated with Diminished Prognosis in Esophageal Cancer and Correlates with Her-2 Expression [Elektronische Daten] [Peter Birner, Bettina Jesch, Julia Friedrich, Martin Riegler, Johannes Zacherl, Michael Hejna, Fritz Wrba, Andrea Schultheis, Sebastian Schoppmann] Background: Carbonic anhydrase IX (CAIX), a transmembrane glycoprotein, seems to play a key role in the adaption of tumor cells to hypoxia. This study was designed to investigate the clinical role of CAIX and its association with Her-2 in a large cohort of adeno- (AC) and squamous cell carcinomas (SCC) of the esophagus and their metastases. Methods: Expression of CAIX and Her-2 was investigated immunohistochemically in formalin fixed, paraffin-embedded tissue from 330 esophageal cancers (182 ACS, 148 SCCs). Corresponding lymph node metastases in 137 cases, distant metastases in 34 cases, and local recurrences in 14 cases were analyzed for CAIX expression. Results: A total of 147 cases (44.5%) showed strong CAIX expression (AC: 46.7%; ACC: 41.9%). CAIX status of the primary tumor influenced CAIX expression in corresponding lymph node metastases (P<0.001, linear regression). High CAIX-expression was an independent prognostic factor for shorter overall and disease-free survival (P≤0.05, Cox regression). Twenty-nine ACs (15.9%) and 6 SCCs (4.1%) showed Her-2 overexpression. In AC, a significant positive correlation between the Her-2 status and CAIX expression was found (P=0.009, chi-square test). Conclusions: High CAIX expression is associated with shorter survival in esophageal cancer, and the hypoxic phenotype seems to be preserved at least during formation of lymph node metastases. Inhibition of CAIX might reduce the ability of tumor cells to establish disseminated disease. In Her-2 overexpressing ACs, blocking of this tyrosine kinase, e.g., by monoclonal antibodies, might induce this effect. Society of Surgical Oncology, 2011 Birner Peter Institute of Pathology, Medical University of Vienna, Vienna, Austria aut Jesch Bettina Department of Surgery, University of Vienna, Vienna, Austria aut Friedrich Julia Institute of Pathology, Medical University of Vienna, Vienna, Austria aut Riegler Martin Department of Surgery, University of Vienna, Vienna, Austria aut Zacherl Johannes Department of Surgery, University of Vienna, Vienna, Austria aut Hejna Michael Department of Oncology, Medical University of Vienna, Vienna, Austria aut Wrba Fritz Institute of Pathology, Medical University of Vienna, Vienna, Austria aut Schultheis Andrea Department of Pathology, Kaiserin-Elisabeth Spital, Vienna, Austria aut Schoppmann Sebastian Department of Surgery, University of Vienna, Vienna, Austria aut Annals of Surgical Oncology Springer-Verlag 18/12(2011-11-01), 3330-3337 1068-9265 18:12<3330 2011 18 10434 https://doi.org/10.1245/s10434-011-1730-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1730-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Birner Peter Institute of Pathology, Medical University of Vienna, Vienna, Austria aut NATIONALLICENCE 700 1- Jesch Bettina Department of Surgery, University of Vienna, Vienna, Austria aut NATIONALLICENCE 700 1- Friedrich Julia Institute of Pathology, Medical University of Vienna, Vienna, Austria aut NATIONALLICENCE 700 1- Riegler Martin Department of Surgery, University of Vienna, Vienna, Austria aut NATIONALLICENCE 700 1- Zacherl Johannes Department of Surgery, University of Vienna, Vienna, Austria aut NATIONALLICENCE 700 1- Hejna Michael Department of Oncology, Medical University of Vienna, Vienna, Austria aut NATIONALLICENCE 700 1- Wrba Fritz Institute of Pathology, Medical University of Vienna, Vienna, Austria aut NATIONALLICENCE 700 1- Schultheis Andrea Department of Pathology, Kaiserin-Elisabeth Spital, Vienna, Austria aut NATIONALLICENCE 700 1- Schoppmann Sebastian Department of Surgery, University of Vienna, Vienna, Austria aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/12(2011-11-01), 3330-3337 1068-9265 18:12<3330 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer