caa a22 4500 44533472X CHVBK 20180317142750.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1245/s10434-011-1798-9 doi (NATIONALLICENCE)springer-10.1245/s10434-011-1798-9 Expression of the Transient Receptor Potential Channel C3 Correlates with a Favorable Prognosis in Patients with Adenocarcinoma of the Lung [Elektronische Daten] [Hajime Saito, Yoshihiro Minamiya, Hiroyuki Watanabe, Naoko Takahashi, Manabu Ito, Hiroshi Toda, Hayato Konno, Masafumi Mitsui, Satoru Motoyama, Jun-ichi Ogawa] Background: The cation channels of the transient receptor potential (TRP) superfamily are implicated in cancer formation; in particular, TRPC3 has been shown to contribute to the progression of human ovarian cancer. However, the relationship between TRP expression and the clinicopathological characteristics of lung cancer and patient prognosis is not well understood. Therefore, we investigated the relationship between TRP expression and the prognosis of patients with adenocarcinoma of the lung. Methods: We used semiquantitative real-time reverse transcription polymerase chain reaction to assess the expression of TRP mRNA in tumor samples from 95 patients with adenocarcinoma of the lung. We then correlated the TRP mRNA levels with clinicopathological factors. We also used immunohistochemical staining to determine the localization of expressed TRP. Results: The 5-year overall and disease-free survival rates among patients expressing higher levels of TRPC3 mRNA were significantly better than the corresponding rates among patients expressing lower levels (P=0.004, P=0.002, respectively, by log-rank test). Multivariate Cox proportional hazard analyses revealed that tumor size (hazard ratio, 2.46; 95% confidence interval [CI], 1.06 to 5.79; P=0.036), n2 (hazard ratio, 3.81; 95% CI, 1.29 to 11.77; P=0.015) and TRPC3 (hazard ratio, 2.71; 95% CI, 1.33 to 5.59; P=0.006) were independent factors affecting the 5-year overall survival rate. Immunohistochemistry showed that the cytoplasm of tumor cells were stained positively for TRPC3. Conclusions: Higher levels of TRPC3 expression in tumor cells are an independent predictor of a better prognosis in patients with adenocarcinoma of the lung. Society of Surgical Oncology, 2011 Saito Hajime Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Minamiya Yoshihiro Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Watanabe Hiroyuki Department of Internal Medicine, Akita University School of Medicine, Akita City, Japan aut Takahashi Naoko Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Ito Manabu Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Toda Hiroshi Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Konno Hayato Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Mitsui Masafumi Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Motoyama Satoru Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Ogawa Jun-ichi Department of Surgery, Akita University School of Medicine, Akita City, Japan aut Annals of Surgical Oncology Springer-Verlag 18/12(2011-11-01), 3377-3383 1068-9265 18:12<3377 2011 18 10434 https://doi.org/10.1245/s10434-011-1798-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1798-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito Hajime Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Minamiya Yoshihiro Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Watanabe Hiroyuki Department of Internal Medicine, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Takahashi Naoko Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Ito Manabu Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Toda Hiroshi Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Konno Hayato Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Mitsui Masafumi Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Motoyama Satoru Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 700 1- Ogawa Jun-ichi Department of Surgery, Akita University School of Medicine, Akita City, Japan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/12(2011-11-01), 3377-3383 1068-9265 18:12<3377 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer