caa a22 4500 445334940 CHVBK 20180317142751.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1245/s10434-010-1313-8 doi (NATIONALLICENCE)springer-10.1245/s10434-010-1313-8 CIP2A is Overexpressed in Non-Small Cell Lung Cancer and Correlates with Poor Prognosis [Elektronische Daten] [Qian-Ze Dong, Yang Wang, Xin-Jun Dong, Zi-Xuan Li, Zhong-Ping Tang, Quan-Ze Cui, En-Hua Wang] Background: Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an oncoprotein inhibiting proteolytic degradation of c-MYC. In this study, we investigated the clinical relevance of CIP2A in NSCLC. Materials and Methods: We analyzed CIP2A mRNA expression in 29 NSCLC tissues using quantitative reverse transcription polymerase chain reaction (RT-QPCR). We also examined the expression of CIP2A protein by immunohistochemistry in 90 lung cancer specimens and correlated its expression with c-MYC expression and clinicopathological parameters. The functional roles of CIP2A in lung cancer cell lines were evaluated by small interfering RNA-mediated depletion of the protein followed by analyses of cell proliferation and invasion. Results: In 29 lung cancer tissues examined, 24 of them (82.7%) exhibited much stronger levels of CIP2A mRNA compared with their corresponding normal tissues. Moreover, CIP2A mRNA expression levels correlated with c-MYC mRNA levels. Furthermore, CIP2A protein was found to be overexpressed in 72.2% of 90 human lung cancer samples and correlated with poor survival (P<0.05). In addition, the CIP2A status was a significant prognostic factor for NSCLC patients (P=0.0136). Depleting CIP2A expression inhibited growth and clonogenic potential in lung cancer cell lines. Conclusions: CIP2A is an oncoprotein overexpressed in NSCLC, and its expression is associated with poor prognosis and malignant cell proliferation. Society of Surgical Oncology, 2010 Dong Qian-Ze Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut Wang Yang Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut Dong Xin-Jun Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut Li Zi-Xuan Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut Tang Zhong-Ping Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut Cui Quan-Ze Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut Wang En-Hua Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut Annals of Surgical Oncology Springer-Verlag 18/3(2011-03-01), 857-865 1068-9265 18:3<857 2011 18 10434 https://doi.org/10.1245/s10434-010-1313-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1313-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Qian-Ze Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut NATIONALLICENCE 700 1- Wang Yang Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut NATIONALLICENCE 700 1- Dong Xin-Jun Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut NATIONALLICENCE 700 1- Li Zi-Xuan Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut NATIONALLICENCE 700 1- Tang Zhong-Ping Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut NATIONALLICENCE 700 1- Cui Quan-Ze Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut NATIONALLICENCE 700 1- Wang En-Hua Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/3(2011-03-01), 857-865 1068-9265 18:3<857 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer