caa a22 4500 445335726 CHVBK 20180317142754.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1245/s10434-011-1653-z doi (NATIONALLICENCE)springer-10.1245/s10434-011-1653-z The Significance of PITX2 Overexpression in Human Colorectal Cancer [Elektronische Daten] [Hajime Hirose, Hideshi Ishii, Koshi Mimori, Fumiaki Tanaka, Ichiro Takemasa, Tsunekazu Mizushima, Masataka Ikeda, Hirofumi Yamamoto, Mitsugu Sekimoto, Yuichiro Doki, Masaki Mori] Purpose: The paired-like homeodomain transcription factor 2 (PITX2) gene encodes a transcription factor controlled by the WNT/Dvl/CTNNB1 and Hedgehog/TGFB pathways in the pathogenesis of colorectal cancer (CRC). Although PITX2 is reportedly involved in various functions, including tissue development by controlling cell growth, its significance in CRC remains unclear. We report our findings regarding abnormal PITX2 expression in human CRC. Methods: PITX2 expression was evaluated in 5 human CRC cell lines and 92 primary CRC samples. Cell growth was evaluated after inhibition of PITX2 expression or after exogenous introduction of PITX2. Results: PITX2 expression was seen in all the five CRC cell lines. The study of tissue samples indicated that PITX2 expression was significantly higher in cancerous tissue than in paired control tissue (P=0.0471). Patients with lower PITX2 expression showed a poorer overall survival rate than those with higher PITX2 expression (P=0.0481). Multivariate analysis demonstrated that PITX2 expression was an independent prognostic factor. Experimental knockdown and introduction of PITX2 also demonstrated that the level of PITX2 expression is inversely associated with cell growth and invasion in vitro. Conclusions: PITX2 expression is significantly related to the biological behavior of CRC cells and appears to be correlated with clinical survival. Thus, this study revealed a previously uncharacterized unique role and significance of PITX2 expression in CRC. Society of Surgical Oncology, 2011 Hirose Hajime Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Ishii Hideshi Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Mimori Koshi Division of Molecular and Surgical Oncology, Department of Molecular and Cellular Biology, Kyushu University, Medical Institute of Bioregulation, Beppu, Oita, Japan aut Tanaka Fumiaki Division of Molecular and Surgical Oncology, Department of Molecular and Cellular Biology, Kyushu University, Medical Institute of Bioregulation, Beppu, Oita, Japan aut Takemasa Ichiro Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Mizushima Tsunekazu Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Ikeda Masataka Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Yamamoto Hirofumi Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Sekimoto Mitsugu Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Doki Yuichiro Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Mori Masaki Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut Annals of Surgical Oncology Springer-Verlag 18/10(2011-10-01), 3005-3012 1068-9265 18:10<3005 2011 18 10434 https://doi.org/10.1245/s10434-011-1653-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1653-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirose Hajime Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Ishii Hideshi Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Mimori Koshi Division of Molecular and Surgical Oncology, Department of Molecular and Cellular Biology, Kyushu University, Medical Institute of Bioregulation, Beppu, Oita, Japan aut NATIONALLICENCE 700 1- Tanaka Fumiaki Division of Molecular and Surgical Oncology, Department of Molecular and Cellular Biology, Kyushu University, Medical Institute of Bioregulation, Beppu, Oita, Japan aut NATIONALLICENCE 700 1- Takemasa Ichiro Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Mizushima Tsunekazu Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Ikeda Masataka Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Yamamoto Hirofumi Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Sekimoto Mitsugu Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Doki Yuichiro Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Mori Masaki Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/10(2011-10-01), 3005-3012 1068-9265 18:10<3005 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer