caa a22 4500 445336218 CHVBK 20180317142756.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1245/s10434-011-1602-x doi (NATIONALLICENCE)springer-10.1245/s10434-011-1602-x MicroRNA Expression as a Predictive Marker for Gemcitabine Response after Surgical Resection of Pancreatic Cancer [Elektronische Daten] [Kenoki Ohuchida, Kazuhiro Mizumoto, Tadashi Kayashima, Hayato Fujita, Taiki Moriyama, Takao Ohtsuka, Junji Ueda, Eishi Nagai, Makoto Hashizume, Masao Tanaka] Background: To improve the prognosis of patients after resection of pancreatic cancer, the most appropriate and efficient treatment should be provided to specific subsets of patients. Our aim was to identify promising microRNAs as markers to predict responses to gemcitabine in patients with resected pancreatic cancer. Methods: Two gemcitabine-resistant pancreatic cancer cell lines were established, and global microRNA expression analyses was performed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Eleven miRNAs were selected as putative predictive markers and analyzed by means of macrodissected formalin-fixed, paraffin-embedded samples obtained from 90 patients with or without gemcitabine treatment after resection of pancreatic cancer. Results: We identified 24 microRNAs whose expression was altered in gemcitabine-resistant cells. qRT-PCR analyses showed that patients with high miR-142-5p and miR-204 expression had significantly longer survival times than those with low miR-142-5p (P=0.0077) and miR-204 (P=0.0054) expression in the gemcitabine-treated group. This was not seen in the nontreated group. Multivariate analyses showed that miR-142-5p expression was an independent prognostic marker only in patients treated with gemcitabine (P=0.034). Conclusions: miR-142-5p is a promising predictive marker for gemcitabine response in patients with resected pancreatic cancer. The Author(s), 2011 Ohuchida Kenoki Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Mizumoto Kazuhiro Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Kayashima Tadashi Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Fujita Hayato Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Moriyama Taiki Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Ohtsuka Takao Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Ueda Junji Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Nagai Eishi Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Hashizume Makoto Department of Advanced Medical Initiatives, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Tanaka Masao Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2381-2387 1068-9265 18:8<2381 2011 18 10434 https://doi.org/10.1245/s10434-011-1602-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1602-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohuchida Kenoki Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Mizumoto Kazuhiro Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kayashima Tadashi Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Fujita Hayato Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Moriyama Taiki Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ohtsuka Takao Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ueda Junji Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nagai Eishi Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Hashizume Makoto Department of Advanced Medical Initiatives, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Tanaka Masao Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2381-2387 1068-9265 18:8<2381 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer