caa a22 4500 445336226 CHVBK 20180317142756.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1245/s10434-011-1572-z doi (NATIONALLICENCE)springer-10.1245/s10434-011-1572-z Identification of the Coexisting HER2 Gene Amplification and Novel Mutations in the HER2 Protein-Overexpressed Mucinous Epithelial Ovarian Cancer [Elektronische Daten] [Wea-Lung Lin, Wu-Hsien Kuo, Fong-Lin Chen, Ming-Yung Lee, Alexandra Ruan, Yeu-Sheng Tyan, Jeng-Dong Hsu, Hung Chiang, Chih-Ping Han] Background: Although overexpression, amplification, and somatic mutation of the HER2 gene have been noted in various types of human cancers, we report here for the first time that novel mutations and amplification of the HER2 gene occurred concomitantly in mucinous epithelial ovarian cancer (EOC). Methods: Twenty-seven tissue microarray samples from EOC patients were analyzed by immunohistochemistry (IHC) with Dako c-erb-B2 antibody and subsequently were examined by fluorescence in situ hybridization (FISH) with the Abbott PathVysion HER2 DNA Probe Kit. HER2 gene, exon 18-24, encoding a tyrosine kinase domain, was analyzed by polymerase chain reaction (PCR) and direct sequencing. Results: The FISH-IHC paired results confirmed 19 concordant negative results and 3 concordant positive results. Moreover, all 4 HER2-amplified cases were of the mucinous type, whereas the remaining 23 HER2-nonamplified cases were of the nonmucinous type. The 4 mucinous EOC cases with HER2 gene amplification were selected and further analyzed for HER2 gene mutations. Data revealed that somatic mutations were present in 2 cases (R970W and E971G), but absent in the other 2 cases. Conclusions: HER2 protein overexpression correlated significantly with HER2 gene amplification in EOC (P=0.027). It is surprising that all 4 cases of mucinous EOC showed HER2 gene amplification confirmed by FISH testing. However, we suppose that increasing the number of cases would possibly modify the results. This study also showed that both HER2 gene amplification and somatic mutations are not mutually exclusive in mucinous EOC. Further studies are warranted to investigate the potential role of anti-HER2 therapy. Society of Surgical Oncology, 2011 Lin Wea-Lung Department of Pathology, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, Taichung, Taiwan aut Kuo Wu-Hsien Department of Internal Medicine, Armed-Force Hualien General Hospital, Hualien, Taiwan aut Chen Fong-Lin Division of Pediatric Cardiology, Department of Pediatrics, Chung Shan Medical University Hospital and School of Medicine, Chung Shan Medical University, Taichung, Taiwan aut Lee Ming-Yung Department of Statistics and Informatics Science, Providence University, Taichung, Taiwan aut Ruan Alexandra Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD, USA aut Tyan Yeu-Sheng Department of Medical Imaging and Radiological Science, Chung-Shan Medical University, Department of Medical Imaging, Chung-Shan Medical University Hospital, Taichung, Taiwan aut Hsu Jeng-Dong Department of Pathology, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, Taichung, Taiwan aut Chiang Hung Taipei Institute of Pathology, Taipei, Taiwan aut Han Chih-Ping Department of Pathology, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, Taichung, Taiwan aut Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2388-2394 1068-9265 18:8<2388 2011 18 10434 https://doi.org/10.1245/s10434-011-1572-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1572-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Wea-Lung Department of Pathology, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Kuo Wu-Hsien Department of Internal Medicine, Armed-Force Hualien General Hospital, Hualien, Taiwan aut NATIONALLICENCE 700 1- Chen Fong-Lin Division of Pediatric Cardiology, Department of Pediatrics, Chung Shan Medical University Hospital and School of Medicine, Chung Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Lee Ming-Yung Department of Statistics and Informatics Science, Providence University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Ruan Alexandra Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Tyan Yeu-Sheng Department of Medical Imaging and Radiological Science, Chung-Shan Medical University, Department of Medical Imaging, Chung-Shan Medical University Hospital, Taichung, Taiwan aut NATIONALLICENCE 700 1- Hsu Jeng-Dong Department of Pathology, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Chiang Hung Taipei Institute of Pathology, Taipei, Taiwan aut NATIONALLICENCE 700 1- Han Chih-Ping Department of Pathology, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2388-2394 1068-9265 18:8<2388 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer