caa a22 4500 445336242 CHVBK 20180317142756.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1245/s10434-011-1588-4 doi (NATIONALLICENCE)springer-10.1245/s10434-011-1588-4 Water: A Simple Solution for Tumor Spillage [Elektronische Daten] [Fumito Ito, Marta Camoriano, Mukund Seshadri, Sharon Evans, John Kane III, Joseph Skitzki] Background: Although often proposed as a means to reduce the harmful consequences of tumor spill, water lavage has yet to be systematically evaluated in relevant in vitro and in vivo models. This study evaluates the mechanisms and utility of a single water lavage to improve the sequelae of tumor spill during laparotomy. Methods: Murine colorectal tumor cell susceptibility to water-induced osmotic lysis was characterized in vitro. A reproducible model of tumor spill was established to recapitulate water or saline lavage during laparotomy. Analyses of tumor volumes calculated from noninvasive imaging were performed. The tumor volumes and survival of mice treated with water, normal saline, or sham laparotomy were assessed. Results: Significant osmotic lysis of cultured murine colorectal cancer cells was observed after a brief exposure to water. Compared to saline or sham laparotomy, water lavage demonstrated superior clinical outcomes with a decrease in tumor burden and concomitant improvement in survival. Conclusions: The use of water lavage during oncologic surgeries to reduce the sequelae of tumor spill is justified and strongly supported by our study. Data from our study raise several concerns regarding the mechanisms and efficacy of saline lavage. Clinically, the use of water lavage during laparotomy would be anticipated to reduce peritoneal disease burden with minimal toxicity or cost. Society of Surgical Oncology, 2011 Ito Fumito Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA aut Camoriano Marta Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY, USA aut Seshadri Mukund Department of Preclinical Imaging Facility, Roswell Park Cancer Institute, Buffalo, NY, USA aut Evans Sharon Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY, USA aut Kane III John Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA aut Skitzki Joseph Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA aut Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2357-2363 1068-9265 18:8<2357 2011 18 10434 https://doi.org/10.1245/s10434-011-1588-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1588-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ito Fumito Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Camoriano Marta Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Seshadri Mukund Department of Preclinical Imaging Facility, Roswell Park Cancer Institute, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Evans Sharon Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Kane III John Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Skitzki Joseph Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2357-2363 1068-9265 18:8<2357 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer