caa a22 4500 445336293 CHVBK 20180317142756.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1245/s10434-011-1597-3 doi (NATIONALLICENCE)springer-10.1245/s10434-011-1597-3 Glucose-Regulated Protein 78 (GRP78) Mediated the Efficacy to Curcumin Treatment on Hepatocellular Carcinoma [Elektronische Daten] [Yu-Jia Chang, Cheng-Jeng Tai, Li-Jen Kuo, Po-Li Wei, Hung-Hua Liang, Tsan-Zon Liu, Weu Wang, Chen-Jei Tai, Yuan-Soon Ho, Chih-Hsiung Wu, Ming-Te Huang] Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells. Society of Surgical Oncology, 2011 Chang Yu-Jia Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan aut Tai Cheng-Jeng Division of Hematology and Oncology, Department of Medicine, Taipei Medical University and Hospital, Taipei, Taiwan aut Kuo Li-Jen Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan aut Wei Po-Li Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan aut Liang Hung-Hua Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan aut Liu Tsan-Zon Translational Research Laboratory, Cancer Center, Taipei Medical University Hospital, Taipei, Taiwan aut Wang Weu Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan aut Tai Chen-Jei Department of Obstetrics and Gynecology, School of Medicine, Taipei Medical University, Taipei, Taiwan aut Ho Yuan-Soon Center of Excellence for Cancer Research, Taipei Medical University, Taipei, Taiwan aut Wu Chih-Hsiung Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan aut Huang Ming-Te Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan aut Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2395-2403 1068-9265 18:8<2395 2011 18 10434 https://doi.org/10.1245/s10434-011-1597-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1597-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chang Yu-Jia Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Tai Cheng-Jeng Division of Hematology and Oncology, Department of Medicine, Taipei Medical University and Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Kuo Li-Jen Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Wei Po-Li Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Liang Hung-Hua Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Liu Tsan-Zon Translational Research Laboratory, Cancer Center, Taipei Medical University Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Wang Weu Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Tai Chen-Jei Department of Obstetrics and Gynecology, School of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Ho Yuan-Soon Center of Excellence for Cancer Research, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Wu Chih-Hsiung Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Huang Ming-Te Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2395-2403 1068-9265 18:8<2395 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer