caa a22 4500 44533634X CHVBK 20180317142756.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1245/s10434-011-1573-y doi (NATIONALLICENCE)springer-10.1245/s10434-011-1573-y Epigenomic Analysis of Aberrantly Methylated Genes in Colorectal Cancer Identifies Genes Commonly Affected by Epigenetic Alterations [Elektronische Daten] [Young-Ho Kim, Han Lee, Seon-Young Kim, Young Yeom, Kyung Ryu, Byung-Hoon Min, Duk-Hwan Kim, Hee Son, Poong-Lyul Rhee, Jae Kim, Jong Rhee, Hee Kim, Ho-Kyung Chun, William Grady, Yong Kim] Background: Determination of the profile of genes that are commonly methylated aberrantly in colorectal cancer (CRC) will have substantial value for diagnostic and therapeutic applications. However, there is limited knowledge of the DNA methylation pattern in CRC. Materials and Methods: We analyzed the methylation profile of 27,578 CpG sites spanning more than 14,000 genes in CRC and in the adjacent normal mucosa with bead-chip array-based technology. Results: We identified 621 CpG sites located in promoter regions and CpG islands that were greatly hypermethylated in CRC compared to normal mucosa. The genes on chromosome 18 showed promoter hypermethylation most frequently. According to gene ontology analysis, the most common biologically relevant class of genes affected by methylation was the class associated with the cadherin signaling pathway. Compared to the genome-wide expression array, mRNA expression was more likely to be downregulated in the genes demonstrating promoter hypermethylation, even though this was not statistically significant. We validated ten CpG sites that were hypermethylated (ADHFE1, BOLL, SLC6A15, ADAMTS5, TFPI2, EYA4, NPY, TWIST1, LAMA1, GAS7) and 2 CpG sites showing hypomethylation (MAEL, SFT2D3) in CRC compared to the normal mucosa in the array studies using pyrosequencing. The methylation status measured by pyrosequencing was consistent with the methylation array data. Conclusions: Methylation profiling based on bead-chip arrays is an effective method for screening aberrantly methylated genes in CRC. In addition, we identified novel methylated genes that are candidate diagnostic or prognostic markers for CRC. Society of Surgical Oncology, 2011 Kim Young-Ho Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Lee Han Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea aut Kim Seon-Young Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea aut Yeom Young Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea aut Ryu Kyung Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Min Byung-Hoon Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Kim Duk-Hwan Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea aut Son Hee Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Rhee Poong-Lyul Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Kim Jae Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Rhee Jong Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Kim Hee Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Chun Ho-Kyung Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut Grady William Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA aut Kim Yong Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea aut Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2338-2347 1068-9265 18:8<2338 2011 18 10434 https://doi.org/10.1245/s10434-011-1573-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1573-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Young-Ho Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Han Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea aut NATIONALLICENCE 700 1- Kim Seon-Young Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea aut NATIONALLICENCE 700 1- Yeom Young Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea aut NATIONALLICENCE 700 1- Ryu Kyung Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Min Byung-Hoon Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Duk-Hwan Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea aut NATIONALLICENCE 700 1- Son Hee Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Rhee Poong-Lyul Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Jae Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Rhee Jong Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Hee Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Chun Ho-Kyung Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Grady William Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA aut NATIONALLICENCE 700 1- Kim Yong Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2338-2347 1068-9265 18:8<2338 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer