caa a22 4500 445336501 CHVBK 20180317142757.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1245/s10434-011-1574-x doi (NATIONALLICENCE)springer-10.1245/s10434-011-1574-x Role of MMP14 Gene Polymorphisms in Susceptibility and Pathological Development to Hepatocellular Carcinoma [Elektronische Daten] [Tzy-Yen Chen, Yi-Ching Li, Yu-Fan Liu, Chiung-Man Tsai, Yi-Hsien Hsieh, Chiao-Wen Lin, Shun-Fa Yang, Chia-Jui Weng] Background: Early detection of hepatocellular carcinoma (HCC) is seldom available because of the lack of reliable markers. Matrix metalloproteinase (MMP) 14 is a cell surface proteinase that displays a broad spectrum of activity against extracellular matrix components and promotes the invasion/metastasis of cells. MMP14 is overexpressed in HCC, and the level is correlated with poor overall survival. The purpose of this study was to examine whether the MMP14 gene polymorphisms are associated with the susceptibility and clinicopathological development of HCC. Methods: A total of 135 patients with HCC and 496 healthy control subjects were recruited. Six single nucleotide polymorphisms (SNPs) of MMP14 genes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genotyping and haplotype-base analysis. Results: A significant (p<0.05) lower risk for HCC was shown in the individuals with MMP14 +6767 G/A and +7096 C/C genotypes compared with those with corresponding wild-type homozygotes; high frequency for anti-hepatitis C virus and cirrhosis positive were shown in the HCC patients with MMP14 +7096 TC/CC genotype after adjusting for other confounding factors. The distribution frequency of −165 T: +221 T: +6727 C: +6767 G: +7096 T: +8153 G haplotype and diplotype was significantly higher in the HCC patients than healthy control subjects. Conclusions: The +6767 and +7096 polymorphic genotypes and haplotype −165 T: +221 T: +6727 C: +6767 G: +7096 T: +8153 G of MMP14 gene might contribute to the prediction of susceptibility and pathological development to HCC. Society of Surgical Oncology, 2011 Chen Tzy-Yen School of Medicine, Chung Shan Medical University, Taichung, Taiwan aut Li Yi-Ching Department of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan aut Liu Yu-Fan Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan aut Tsai Chiung-Man Chest Hospital, Department of Health, Executive Yuan, Tainan, Taiwan aut Hsieh Yi-Hsien Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan aut Lin Chiao-Wen Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan aut Yang Shun-Fa Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan aut Weng Chia-Jui Graduate Institute of Applied Science of Living, Tainan University of Technology, Tainan City, Taiwan aut Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2348-2356 1068-9265 18:8<2348 2011 18 10434 https://doi.org/10.1245/s10434-011-1574-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-011-1574-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Tzy-Yen School of Medicine, Chung Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Li Yi-Ching Department of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Liu Yu-Fan Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Tsai Chiung-Man Chest Hospital, Department of Health, Executive Yuan, Tainan, Taiwan aut NATIONALLICENCE 700 1- Hsieh Yi-Hsien Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Lin Chiao-Wen Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Yang Shun-Fa Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan aut NATIONALLICENCE 700 1- Weng Chia-Jui Graduate Institute of Applied Science of Living, Tainan University of Technology, Tainan City, Taiwan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/8(2011-08-01), 2348-2356 1068-9265 18:8<2348 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer