caa a22 4500 445338288 CHVBK 20180317142803.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1245/s10434-010-1273-z doi (NATIONALLICENCE)springer-10.1245/s10434-010-1273-z Reduced Expression of Reelin ( RELN ) Gene Is Associated With High Recurrence Rate of Hepatocellular Carcinoma [Elektronische Daten] [Yukiyasu Okamura, Shuji Nomoto, Mitsuro Kanda, Masamichi Hayashi, Yoko Nishikawa, Tsutomu Fujii, Hiroyuki Sugimoto, Shin Takeda, Akimasa Nakao] Background: Hepatocellular carcinoma (HCC) is one of the world's top five causes of cancer-related deaths. Current treatments available ameliorate HCC; however, current therapy fails to completely treat and prevent HCC, as shown by its high recurrence rate. Recently developed genome-wide gene-expression profile analyses can now robustly detect many candidate genes that are modified by HCC. Here we attempt to identify novel genes displaying altered gene expression profiles when comparing healthy tissue with HCC by means of a double-combination array previously developed. Methods: Double-combination array analysis of gene expression profiles and single nucleotide polymorphism arrays were performed on each HCC tissue sample. Subsequently, samples from 48 HCC patients were subjected to quantitative real-time reverse transcription polymerase chain reaction and methylation-specific polymerase chain reaction. Results: The reelin (RELN) gene was detected as a pertinent tumor suppressor gene by means of this method. Of the 48 clinical samples obtained, 34 (79.2%) displayed reduced RELN expression in tumor tissue, and the expression level of tumor tissues clearly reduced compared with that of corresponding normal tissues (P=0.002). Eighteen (37.5%) of 48 tumor tissues were found to be hypermethylated on the RELN gene promoter. Moreover, analysis of clinical data revealed an inverse correlation between RELN expression and HCC recurrence. Conclusions: The present study indicates that our in-house double-combination array is an effective and convenient technique in detecting novel genes with altered expression in disease. We suggest RELN is a key regulatory gene associated with the recurrence of HCC. Society of Surgical Oncology, 2010 Okamura Yukiyasu Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Nomoto Shuji Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Kanda Mitsuro Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Hayashi Masamichi Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Nishikawa Yoko Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Fujii Tsutomu Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Sugimoto Hiroyuki Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Takeda Shin Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Nakao Akimasa Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut Annals of Surgical Oncology Springer-Verlag 18/2(2011-02-01), 572-579 1068-9265 18:2<572 2011 18 10434 https://doi.org/10.1245/s10434-010-1273-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1273-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okamura Yukiyasu Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 700 1- Nomoto Shuji Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 700 1- Kanda Mitsuro Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 700 1- Hayashi Masamichi Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 700 1- Nishikawa Yoko Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 700 1- Fujii Tsutomu Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 700 1- Sugimoto Hiroyuki Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 700 1- Takeda Shin Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 700 1- Nakao Akimasa Department of Surgery II, Graduate School and Medicine, University of Nagoya, Nagoya, Japan aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/2(2011-02-01), 572-579 1068-9265 18:2<572 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer