caa a22 4500 445338326 CHVBK 20180317142803.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1245/s10434-010-1226-6 doi (NATIONALLICENCE)springer-10.1245/s10434-010-1226-6 Prognostic Significance of the Alterations of the G1-S Checkpoint in Localized Leiomyosarcoma of the Peripheral Soft Tissue [Elektronische Daten] [John Panelos, Giovanni Beltrami, Guido Scoccianti, Rodolfo Capanna, Milena Paglierani, Monica Pepi, Daniela Massi, Alessandro Franchi] Background: The aim of this study was to investigate the expression of cell cycle regulators p53, p16, cyclin-D1, and retinoblastoma (Rb) gene protein in leiomyosarcoma of the peripheral soft in order to identify expression profiles potentially useful for clinical prognostic purposes. Materials and Methods: A tissue microarray representing 70 localized leiomyosarcomas of the limbs and limb girdles was created with 3 representative cores from each tumor. Immunohistochemical staining was performed for p53, p16, cyclin-D1, and Rb using standard techniques. Staining was scored as either absent-low (<20% of neoplastic cells) or moderate-diffuse (≥20%). Outcome analysis was performed for local recurrence-free survival (LFS), metastatic disease-free survival (MDFS), and disease-specific survival (DSS). Results: Kaplan-Meier analysis of survival revealed that no single alteration of the factors examined was associated with outcome, but tumors showing concomitant alteration of p16 and p53 were characterized by reduced MDFS and DSS (P=0.01 and P<0.001, respectively). In addition, patients who received adjuvant therapy consisting of radiotherapy alone or radiotherapy and chemotherapy had a better DSS than those receiving surgery alone or surgery and chemotherapy (P=0.05). In multivariate analysis, altered p16/p53 remained the only parameter predictive of MDFS and DSS (P=0.048, hazard ratio [HR]=2.488, 95% confidence interval [95% CI] 1.006-5.116; P=0.043, HR=2.498, 95% CI 1.029-5.909, respectively). Conclusions: Accumulation of cell cycle alterations represents a prognostic indicator in localized soft tissue leiomyosarcoma, and in particular altered p16/p53 expression is associated with an unfavorable prognosis. This may help the clinical management of patients with leiomyosarcomas. Society of Surgical Oncology, 2010 Panelos John Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut Beltrami Giovanni Department of Orthopedic Oncology, Azienda Ospedaliera Universitaria Careggi, Florence, Italy aut Scoccianti Guido Department of Orthopedic Oncology, Azienda Ospedaliera Universitaria Careggi, Florence, Italy aut Capanna Rodolfo Department of Orthopedic Oncology, Azienda Ospedaliera Universitaria Careggi, Florence, Italy aut Paglierani Milena Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut Pepi Monica Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut Massi Daniela Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut Franchi Alessandro Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut Annals of Surgical Oncology Springer-Verlag 18/2(2011-02-01), 566-571 1068-9265 18:2<566 2011 18 10434 https://doi.org/10.1245/s10434-010-1226-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1245/s10434-010-1226-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Panelos John Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut NATIONALLICENCE 700 1- Beltrami Giovanni Department of Orthopedic Oncology, Azienda Ospedaliera Universitaria Careggi, Florence, Italy aut NATIONALLICENCE 700 1- Scoccianti Guido Department of Orthopedic Oncology, Azienda Ospedaliera Universitaria Careggi, Florence, Italy aut NATIONALLICENCE 700 1- Capanna Rodolfo Department of Orthopedic Oncology, Azienda Ospedaliera Universitaria Careggi, Florence, Italy aut NATIONALLICENCE 700 1- Paglierani Milena Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut NATIONALLICENCE 700 1- Pepi Monica Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut NATIONALLICENCE 700 1- Massi Daniela Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut NATIONALLICENCE 700 1- Franchi Alessandro Department of Human Pathology and Oncology, University of Florence Medical School, Florence, Italy aut NATIONALLICENCE 773 0- Annals of Surgical Oncology Springer-Verlag 18/2(2011-02-01), 566-571 1068-9265 18:2<566 2011 18 10434 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer